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Gene Review:

infB - translation initiation factor IF-2

Escherichia coli O157:H7 str. Sakai

Bremaud, L. et al., Laursen, B.S. et al., Shazand, K. et al., Mortensen, K.K. et al., Hubert, M. et al., et al.

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Disease relevance of infB

A functional copy of the infB gene is supplied in trans by a thermosensitive lysogenic lambda phage integrated at att lambda [1].
These two proteins cross-reacted with antiserum to E. coli IF2 alpha and were able to complement in vivo an E. coli infB gene disruption [2].
Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2 [2].
The infB gene (ca. 3.16 kb) encodes a 1,054-residue polypeptide with extensive homology within its G domain and C terminus with the equivalent regions of IF2s from Escherichia coli, Bacillus subtilis, Bacillus stearothermophilus, and Streptococcus faecium [3].
We have isolated the structural gene for translation initiation factor IF2 (infB) from the myxobacterium Myxococcus xanthus [4].

High impact information on infB

The expression of the nusA-infB operon has been investigated using an in vitro system based on the formation of the first dipeptide of the gene product [5].
Bacterial translation initiation factor IF2 was localized on the ribosome by rRNA cleavage using free Cu(II):1,10-orthophenanthroline [6].
The mutations causing the suppression phenotype are located within infB [7].
The only variations from the wild-type (wt) infB found in the two mutant strains are a replacement of Asp409 with Glu in strain IQ489 and an insertion of Gly between Ala421 and Gly422 in strain IQ490 [7].
Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA) [8].

Biological context of infB

The N-terminal amino acid sequence of the two proteins was determined and the results confirmed that the two forms were IF2 alpha and -beta, both encoded by the infB gene [9].
In this system the expression of infB is under the control of the strong promoter lambda PL and the cells carry the plasmid pcI857, which contains a thermosensible lambda cI repressor [10].
Evidence for autoregulation of the nusA-infB operon of Escherichia coli [11].

References

A severely truncated form of translational initiation factor 2 supports growth of Escherichia coli. Laalami, S., Putzer, H., Plumbridge, J.A., Grunberg-Manago, M. J. Mol. Biol. (1991) [Pubmed]
Isolation and molecular genetic characterization of the Bacillus subtilis gene (infB) encoding protein synthesis initiation factor 2. Shazand, K., Tucker, J., Chiang, R., Stansmore, K., Sperling-Petersen, H.U., Grunberg-Manago, M., Rabinowitz, J.C., Leighton, T. J. Bacteriol. (1990) [Pubmed]
Translation initiation factor IF2 of the myxobacterium Stigmatella aurantiaca: presence of a single species with an unusual N-terminal sequence. Bremaud, L., Laalami, S., Derijard, B., Cenatiempo, Y. J. Bacteriol. (1997) [Pubmed]
Initiation factor 2 of Myxococcus xanthus, a large version of prokaryotic translation initiation factor 2. Tiennault-Desbordes, E., Cenatiempo, Y., Laalami, S. J. Bacteriol. (2001) [Pubmed]
In vitro expression of the Escherichia coli nusA-infB operon. Cenatiempo, Y., Deville, F., Brot, N., Weissbach, H. J. Biol. Chem. (1987) [Pubmed]
The translation initiation functions of IF2: targets for thiostrepton inhibition. Brandi, L., Marzi, S., Fabbretti, A., Fleischer, C., Hill, W.E., Gualerzi, C.O., Stephen Lodmell, J. J. Mol. Biol. (2004) [Pubmed]
Characterization of mutations in the GTP-binding domain of IF2 resulting in cold-sensitive growth of Escherichia coli. Laursen, B.S., Siwanowicz, I., Larigauderie, G., Hedegaard, J., Ito, K., Nakamura, Y., Kenney, J.M., Mortensen, K.K., Sperling-Petersen, H.U. J. Mol. Biol. (2003) [Pubmed]
Organization of the Escherichia coli chromosome around the genes for translation initiation factor IF2 (infB) and a transcription termination factor (nusA). Plumbridge, J.A., Springer, M. J. Mol. Biol. (1983) [Pubmed]
Tandem translation of Bacillus subtilis initiation factor IF2 in E. coli. Over-expression of infBB.su in E. coli and purification of alpha- and beta-forms of IF2B.su. Hubert, M., Nyengaard, N.R., Shazand, K., Mortensen, K.K., Lassen, S.F., Grunberg-Manago, M., Sperling-Petersen, H.U. FEBS Lett. (1992) [Pubmed]
Superexpression and fast purification of E coli initiation factor IF2. Mortensen, K.K., Nyengaard, N.R., Hershey, J.W., Laalami, S., Sperling-Petersen, H.U. Biochimie (1991) [Pubmed]
Evidence for autoregulation of the nusA-infB operon of Escherichia coli. Nakamura, Y., Plumbridge, J., Dondon, J., Grunberg-Manago, M. Gene (1985) [Pubmed]

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