Gene Review:
aksA - trans-homoaconitate synthase
Nostoc sp. PCC 7120
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Disease relevance of nifV1
- In contrast, the Anabaena strain 7120 leuA gene did not complement the nifV mutation of R229I efficiently [1].
High impact information on nifV1
- Heterologous complementation of the Rhodobacter capsulatus nifV mutant R229I by the Anabaena strain 7120 nifV gene corroborated the assumption that Anabaena strain 7120 nifV also encodes a homocitrate synthase [1].
- The nifV gene in Anabaena strain 7120 was interrupted by an interposon insertion (mutant strain BMB105) and by a plasmid integration via a single crossover with a nifV internal fragment as a site for recombination (mutant strain BMB106) [1].
Chemical compound and disease context of nifV1
- The nifV and leuA genes, which encode homocitrate synthase and alpha-isopropylmalate synthase, respectively, were cloned from the filamentous cyanobacterium Anabaena sp. strain PCC 7120 by a PCR-based strategy [1].
Analytical, diagnostic and therapeutic context of nifV1
- Sequence analysis of cloned PCR products confirmed the presence of two different nifV-like DNA fragments, which were subsequently used as nifV- and leuA-specific probes, respectively, to clone XbaI fragments of 2.1 kbp (pOST4) and 2.6 kbp (pOST2) [1].
References
- Identification and characterization of the nifV-nifZ-nifT gene region from the filamentous cyanobacterium Anabaena sp. strain PCC 7120. Stricker, O., Masepohl, B., Klipp, W., Böhme, H. J. Bacteriol. (1997) [Pubmed]
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