The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

SUPT16H  -  suppressor of Ty 16 homolog (S. cerevisiae)

Homo sapiens

Synonyms: CDC68, Chromatin-specific transcription elongation factor 140 kDa subunit, FACT, FACT 140 kDa subunit, FACT complex subunit SPT16, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on SUPT16H

  • Over-expression of ySpt16p/Cdc68p suppressed the phenotype of cold sensitivity of the yTFIIE-alpha-cs mutant strain, and in vitro binding assays revealed that yTFIIE-alpha-cs mutant protein showed diminished binding affinity to ySpt16p/Cdc68p [1].
Role of SUPT16H in transcription
  • SUPT16H interacts with SSRP1 and cooperates with monoubiquitinated histone H2B (by RNF20 and RNF40) during transcriptional elongation [2].
  • SUPT16H and SSRP1 make up the Facilitates Chromatin Transcription (FACT) histone chaperone complex [3].
  • The SUPT16H and SSRP1-containing FACT histone chaperone complex functions to increase transcription by opening chromatin through the displacement of an H2A-H2B dimer from the nucleosome octamer [4].

Role of SUPT16H in DNA repair

  • SUPT16H recognizes the phosphorylated histone variant H2AX (γH2AX) and catalyzes the exchange of an H2AX-H2B dimer in vitro [5].
  • SUPT16H histone chaperone activity is regulated by ADP-ribosylation [5] [6].


  1. Functional interaction of general transcription initiation factor TFIIE with general chromatin factor SPT16/CDC68. Kang, S.W., Kuzuhara, T., Horikoshi, M. Genes Cells (2000) [Pubmed]
  2. Histone H2B monoubiquitination functions cooperatively with FACT to regulate elongation by RNA polymerase II. Pavri, R., Zhu, B., Li, G., Trojer, P., Mandal, S., Shilatifard, A., Reinberg, D. Cell. (2006) [Pubmed]
  3. The chromatin-specific transcription elongation factor FACT comprises human SPT16 and SSRP1 proteins. Orphanides, G., Wu, W.H., Lane, W.S., Hampsey, M., Reinberg, D. Nature. (1999) [Pubmed]
  4. FACT facilitates transcription-dependent nucleosome alteration. Belotserkovskaya, R., Oh, S., Bondarenko, V.A., Orphanides, G., Studitsky, V.M., Reinberg, D. Science. (2003) [Pubmed]
  5. FACT-mediated exchange of histone variant H2AX regulated by phosphorylation of H2AX and ADP-ribosylation of Spt16. Heo, K., Kim, H., Choi, S.H., Choi, J., Kim, K., Gu, J., Lieber, M.R., Yang, A.S., An, W. Mol. Cell. (2008) [Pubmed]
  6. Modulation of nucleosome-binding activity of FACT by poly(ADP-ribosyl)ation. Huang, J.Y., Chen, W.H., Chang, Y.L., Wang, H.T., Chuang, W.T., Lee, S.C. Nucleic. Acids. Res. (2006) [Pubmed]
WikiGenes - Universities