Disease relevance of SSRP1

• Our results confirm that anti-SSRP1 Abs are associated with but not specific for the lupus disease [1].
• The pain of pulmonary hypertension. Fact or fancy [2]?
• "Food allergy": Fact or Fiction [3]?
• Trypanosomiasis and Tsetse Control with Insecticidal Pour-ons- Fact and Fiction [4]?
• Simple carbohydrates and obesity: Fact, Fiction and Future [5].

Psychiatry related information on SSRP1

• Patient and Family Fact Sheet. Tourette syndrome [6].
• Featured CME topic: dementia. Fact sheets [7].
• Psychometric evaluation of the Functional Assessment of Cancer Therapy/Gynecologic Oncology Group-Neurotoxicity (Fact/GOG-Ntx) questionnaire for patients receiving systemic chemotherapy [8].

High impact information on SSRP1

• A DNA damage-induced p53 serine 392 kinase complex contains CK2, hSpt16, and SSRP1 [9].
• For activity, CDKs require association with a cyclin and phosphorylation by a separate protein kinase at a conserved threonine residue (T160 in CDK2) [10].
• Analysis of HeLa cells synchronized at various cell cycle stages indicates that CDK2 phosphorylation on T160 increases during S phase and G2, when CDK2 is most active [11].
• Analysis of site-directed mutant proteins, expressed by transient transfection of COS cells, demonstrates that the two major phosphorylation sites are Tyr15 (Y15) and Thr160 (T160) [11].
• The SSRP1 gene maps to human chromosome 11q12 [12].

Biological context of SSRP1

• The HMG domain protein SSRP1/PREIIBF is involved in activation of the human embryonic beta-like globin gene [13].
• Here we extend this work by investigating CK2 regulation of SSRP1 function through phosphorylation [14].
• Mutagenesis of the three serines revealed that serine 510 was more important for the regulation of SSRP1 DNA-binding activity [14].
• Immunocytochemical analyses indicated that CHD1 and SSRP1 colocalize in both mammalian nuclei and Drosophila polytene chromosomes [15].
• The structure-specific recognition protein SSRP1, initially isolated from expression screening of a human B-cell cDNA library for proteins that bind to cisplatin (cis-diamminedichloroplatinum(II))-modified DNA, contains a single DNA-binding high mobility group (HMG) domain [16].

Anatomical context of SSRP1

• Expression analysis in a variety of tissues and cisplatin-resistant cell lines and the inability of cisplatin to induce the message in HeLa cells argue against a direct link between SSRP1 mRNA levels and the response of cells to the drug [12].
• Moreover, we show that SSRP1/PREIIBF lacks a classical activation domain but that binding by this protein to PRE II is required for activation of a minimal promoter in stable erythroid cell lines [13].
• Collectively, these findings indicate a role for SSRP1 in the UV response of eukaryotic cells [17].
• To determine whether HMG-1 box protein T160 plays a role in virus replication, we employed an antisense strategy to inhibit its expression in NIH 3T3 cells [18].
• Tourniquet-induced restriction of the quadriceps muscle mechanism. Fact or fiction [19]?

Associations of SSRP1 with chemical compounds

• Using acetic acid urea polyacrylamide gel electrophoresis and mass spectrometry, we have examined the phosphorylation of maize SSRP1 by protein kinase CK2 alpha [17].
• Differential chromatin association and nucleosome binding of the maize HMGA, HMGB, and SSRP1 proteins [20].
• Replacement of T160 with alanine abolishes the kinase activity of CDK2, indicating that phosphorylation at this site (as in CDC2) is required for kinase activity [11].
• Viscosupplementation therapy with intra-articular hyaluronic acid. Fact or fantasy [21]?
• Three Careers "Fact Sheets" include information on CE and BMET job titles, job descriptions, and certification [22].

Other interactions of SSRP1

• These results reveal an important role for SSRP1 as a coregulator of SRF-dependent transcription in mammalian cells [23].
• SSRP1/PREIIBF may serve an architectural function by helping to coordinate the assembly of a multiprotein complex required for stage-specific regulation of the human epsilon-globin gene [13].
• This was confirmed by treatment of chromatin with micrococcal nuclease, demonstrating that the HMGA, HMGB2/3, and SSRP1 proteins are enriched in the highly nuclease-sensitive fraction of chromatin, which is likely to be transcriptionally competent [20].

Analytical, diagnostic and therapeutic context of SSRP1

• The affinity and specificity of FACT, SSRP1, and the isolated HMG domain of SSRP1 for cisplatin-damaged DNA were investigated by gel mobility shift assays [16].
• The 30th Sir Frederick Bartlett lecture. Fact, artefact, and myth about blindsight [24].
• Functional neck dissection: Fact and fiction [25].
• Human cloning. Fact or fiction [26].
• Orthodontics. Part 7: Fact and fantasy in orthodontics [27].

References