Disease relevance of hemF

• The Alcaligenes eutrophus hemN gene encoding the oxygen-independent coproporphyrinogen III oxidase, is required for heme biosynthesis during anaerobic growth [1].
• The hemZ gene, encoding a 501-amino-acid protein with a calculated molecular mass of 57,533 Da, complemented a Salmonella typhimurium hemF hemN double mutant under aerobic and anaerobic growth conditions [2].

High impact information on hemF

• B. subtilis hemN functionally complemented two Salmonella typhimurium hemF hemN double mutants under aerobic and anaerobic conditions [3].
• Moreover, B. subtilis hemY did not substitute for hemF hemN deficiency in S. typhimurium [3].
• A single open reading frame was identified as the hemN gene on the basis of its interruption by the insertion mutation and plasmid complementation studies of hemF hemN double mutants [4].
• Here, we report the cloning of the S. typhimurium hemF gene and its DNA sequence [5].
• The hemF operon is transcribed clockwise with respect to the genetic map [5].

Other interactions of hemF

• On the basis of genetic studies, we have suggested that this reaction may be catalyzed by either of two different enzymes, an oxygen-dependent one encoded by hemF or an oxygen-independent enzyme encoded by hemN [5].

References