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Gene Review

pseT  -  polynucleotide kinase

Enterobacteria phage T4

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Disease relevance of pseT

  • Induction of a lambda E-W-S-cI857 prophage in which the pseT gene can be transcribed from the late lambda promoter, PR1, leads to greater than 100-fold amplification of pnk activity; pnk comprises approximately 7% of the total soluble cell protein [1].
  • The purification of polynucleotide kinase from Escherichia coli infected by two different mutants in the T4 polynucleotide kinase (pseT) gene is described [2].
  • The gene, pseT, of coliphage T4 which encodes polynucleotide kinase (PNK) was cloned directly into an expression plasmid using the polymerase chain reaction [3].

High impact information on pseT


  1. T4 polynucleotide kinase; cloning of the gene (pseT) and amplification of its product. Midgley, C.A., Murray, N.E. EMBO J. (1985) [Pubmed]
  2. Isolation and characterization of two mutant forms of T4 polynucleotide kinase. Soltis, D.A., Uhlenbeck, O.C. J. Biol. Chem. (1982) [Pubmed]
  3. Cloning of coliphage-T4 gene pseT and high-level synthesis of polynucleotide kinase in Escherichia coli. Campos, M., Ortega, M., Padrón, G., Estrada, M.P., de la Fuente, J., Herrera, L. Gene (1991) [Pubmed]
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