Disease relevance of Coliphages

• The length of double-stranded coliphage lambda DNA, as determined by electron microscopy using the benzyldimethylalkyl ammonium chloride technique, depends on the mode of dehydration [1].
• Procoat, the precursor form of the major coat protein of coliphage M13, assembles into the Escherichia coli inner membrane and is cleaved to mature coat protein by leader peptidase [2].
• The Cro-like Apl repressor of coliphage 186 is required for prophage excision and binds near the phage attachment site [3].
• The dual role of Apl in prophage induction of coliphage 186 [4].
• DNA from polyoma virus and from coliphages T4 and T7 served as reference markers in estimating the molecular weight of MSP2 DNA from sedimentation in sucrose gradients [5].

High impact information on Coliphages

• Coliphage N4 virion-encapsidated, DNA-dependent RNA polymerase (vRNAP) is inactive on double-stranded N4 DNA; however, denatured promoter-containing templates are accurately transcribed [6].
• Thermosensitivity of a DNA recognition site: activity of a truncated nutL antiterminator of coliphage lambda [7].
• Plasmids were constructed with and without interposition of the rho-independent coliphage T7 'early' terminator between a promoter and galK [8].
• Compounds 3a, 3b, and 3c have been synthesized, and the interaction of distamycin A, 2, 3a, 3b, and 3c with calf thymus DNA, poly(dA-dT), poly(dG-dC), poly(dI-dC), pBR322 superhelical plasmid DNA, and, in the case of 3b, T4 coliphage DNA have been studied [9].
• Tum function, encoded near the right-hand end of the coliphage 186 chromosome, is under the control of promoter p95 [10].

Chemical compound and disease context of Coliphages

• Acquisition of a determinant for chloramphenicol resistance by coliphage lambda [11].
• T-even coliphages have 5-hydroxymethylcytosine in their DNA instead of cytosine [12].
• Single-stranded DNA of coliphage M13mp8 was treated with the oxidizing agent, KMnO4, under conditions that selectively form cis-5,6-dihydro-5,6-dihydroxythymine (thymine glycol) [13].
• The primary structures of the major coat proteins from If1 and Ike filmentous coliphages have been determined by automated Edman degradation before and after cyanogen bromide cleavage and by manual sequencing of certain tryptic peptides [14].
• Sequence of the A-protein of coliphage MS2. I. Isolation of A-protein, determination of the NH2- and COOH-terminal sequences, isolation and amino acid sequence of the tryptic peptides [15].

Biological context of Coliphages

• The role of the primosome in unwinding duplex DNA strands was investigated in the coliphage phi X174 SS(c)----replicative form DNA replication reaction in vitro, which requires the E. coli single-stranded DNA binding protein, the primosomal proteins, and the DNA polymerase III holoenzyme [16].
• The cI gene of coliphage 186 maintains lysogeny and confers immunity to 186 infection by repressing the major early promoter, p(R), and the promoter for the late transcription activator gene, p(B) [17].
• RNA coliphage SP was propagated for several generations on a host expressing an inhibitory antisense RNA complementary to bases 31-270 of the positive-stranded genome [18].
• Nucleotide sequence and analysis of the coliphage T3 S-adenosylmethionine hydrolase gene and its surrounding ribonuclease III processing sites [19].
• Using a 'one-hybrid-system' we found that the DnaA N-terminus can functionally replace the dimerization domain of coliphage lambda cl repressor: a lambdacl-DnaA chimeric protein inhibits lambda plasmid replication as efficiently as lambdacI repressor [20].

Anatomical context of Coliphages

• In the RNA coliphage SP, the gene for the maturation protein was found to be the best target for this type of immune system; mRNA-interfering complementary RNAs specific to the genes for coat protein and replicase were less effective in preventing infection [21].
• The ability of the modified ribosomes to form an initiation complex as measured by the A-U-G or coliphage MS2 RNA dependent binding of (14-C)-fmet-tRNA-fmet was also impaired, as was their ability to incorporate (14-C) lysine into protein with MS2 RNA as messenger [22].
• Similar concentrations of both larger and smaller particles, such as polystyrene latex spheres and coliphage f2, also exhibited a low degree of interaction, viz., 17 to 37%, with microtubules [23].
• It is concluded that in K. pneumoniae adherence to epithelial cells is mediated by the receptor for coliphages T7 (and T3), which in turn recognizes D-mannose in the receptors it binds [24].
• The major coat protein of coliphage M13 is also bound to the cytoplasmic membrane (prior to phage assembly) but with its antigenic sites exposed to the exterior of the cell [25].

Gene context of Coliphages

• The carboxyl-terminal 133 amino acids of the c2 repressor are homologous to the carboxyl-terminal sequence of the coliphage lambda cI repressor [26].
• The sieB gene of coliphage lambda was also identified [27].
• The deduced amino acid sequence of HP1 integrase has remarkable similarity to that deduced for the integrase of coliphage 186 [28].
• A coliphage M13 chimer containing the Saccharomyces cerevisiae TRP1 gene and ARS1 replication origin (mPY2) was grown on an ung- dut- strain of Escherichia coli [29].
• Site-specific recombination in human cells catalyzed by the wild-type integrase protein of coliphage HK022 [30].

Analytical, diagnostic and therapeutic context of Coliphages

• The activity of the Integrase (Int) protein encoded by coliphage HK022 was tested in a human cell culture [30].

References