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Gene Review:

gag - gag protein

Jaagsiekte sheep retrovirus

Yasuda, J. et al., Conte, M.R. et al., Konvalinka, J. et al., Hong, S. et al., Sakalian, M. et al.

Sakalian, Dittmer, Gandy, Rapp, Zábranský, Hunter,

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Disease relevance of gag

In type B and D retroviruses, the Gag protein pre-assembles before association with the membrane, whereas in type C retroviruses (lentiviruses, BLV/HTLV group) Gag is targeted efficiently to the plasma membrane, where the particle formation occurs [1].
Overexpression of BUL1 enhanced virus particle release, while a BUL1 mutant in which a W to G substitution was introduced into a WW domain, W791G, lost the ability to bind to the viral Gag protein and abolished its ability to mediate virus budding [2].
In contrast, the human immunodeficiency virus (HIV) Gag protein is incapable of assembly in parallel assays [3].
We found that two mutants with premature termination codons that remove half or all of the p4 domain produced lower levels of stable Gag protein and of self-assembled capsids [4].
In contrast to the requirement for high ionic strength for peptide cleavage reported previously, we found that Gag protein cleavage by HIV-1 PR proceeded best at low ionic strength, for both of the protein substrates tested [5].

References

The three-dimensional solution structure of the matrix protein from the type D retrovirus, the Mason-Pfizer monkey virus, and implications for the morphology of retroviral assembly. Conte, M.R., Klikova, M., Hunter, E., Ruml, T., Matthews, S. EMBO J. (1997) [Pubmed]
Functional involvement of a novel Nedd4-like ubiquitin ligase on retrovirus budding. Yasuda, J., Hunter, E., Nakao, M., Shida, H. EMBO Rep. (2002) [Pubmed]
The Mason-Pfizer monkey virus internal scaffold domain enables in vitro assembly of human immunodeficiency virus type 1 Gag. Sakalian, M., Dittmer, S.S., Gandy, A.D., Rapp, N.D., Zábranský, A., Hunter, E. J. Virol. (2002) [Pubmed]
Type D retrovirus Gag polyprotein interacts with the cytosolic chaperonin TRiC. Hong, S., Choi, G., Park, S., Chung, A.S., Hunter, E., Rhee, S.S. J. Virol. (2001) [Pubmed]
Proteolytic processing of particle-associated retroviral polyproteins by homologous and heterologous viral proteinases. Konvalinka, J., Heuser, A.M., Hruskova-Heidingsfeldova, O., Vogt, V.M., Sedlacek, J., Strop, P., Kräusslich, H.G. Eur. J. Biochem. (1995) [Pubmed]

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