High impact information on vit-5

• Stable transgenic strains were isolated expressing a gene in which 69 nucleotides of a vit-5 intron, including the 3' splice site, were inserted into the 5' untranslated region of a vit-2/vit-6 fusion gene [1].
• The first consists of two polypeptides with an Mr of about 170,000 (yp170A and yp170B) encoded by a family of five closely related genes called vit-1 through vit-5 [2].
• Here we report the complete nucleotide sequence of one of these genes, vit-5 [3].
• However, the vit-5-encoded protein does not contain a serine-rich sequence related to the vertebrate vitellin, phosvitin [3].
• Caenorhabditis elegans vitellogenins are encoded by a family of six genes, one of which, vit-5, has been previously sequenced and shown to be surprisingly closely related to the vertebrate vitellogenin genes [4].

Biological context of vit-5

• Here we report an alignment of the amino acid sequences of vitellogenins from frog and chicken with those from three C. elegans genes: vit-5 and two newly sequenced genes, vit-2 and vit-6 [4].
• The four introns of vit-6 are all in different places from the four introns of vit-5, but three of these eight positions are identical or close to intron locations in the vertebrate vitellogenin genes [4].

Associations of vit-5 with chemical compounds

• After progesterone treatment (10(-7) M), the vit-5 and vit-6 were down-regulated and vit-1 up-regulated (3.59-fold) [5].

Other interactions of vit-5

• Comparison of portions of the coding sequence indicates that vit-6 is distantly related to the vit-1 through vit-5 gene family [2].

References