Disease relevance of Pde4b

• Analyses of TCRB rearrangements substantiate a profound deficit in recombination signal sequence joining in SCID foals: implications for the role of DNA-dependent protein kinase in V(D)J recombination [1].
• A profound alteration of blood TCRB repertoire allows prediction of cerebral malaria [2].

High impact information on Pde4b

• Pre-TCR expression on developing thymocytes allows cells with productive TCRB gene rearrangements to further differentiate [3].
• After establishing a short-term T cell line, cells were 1) stained with mAbs for flow cytometric analysis, 2) analyzed for TCRB chain transcript expression, and 3) used as effector cells for cytotoxicity and proliferation [4].
• In one case, two expanded TCRB clones could be identified that were persistent in the bile duct over a 1-yr period [4].

Analytical, diagnostic and therapeutic context of Pde4b

• This was confirmed by cloning and random sequencing of PCR amplification products using TCRBV region family-specific primers; TCRB chain sequences were reiterated in all transcripts analyzed [4].

References