High impact information on FOXF2

• Activation domain 1 consists of the most C-terminal 23 amino acids of FREAC-2 and contains a sequence motif conserved in an activation domain of another forkhead protein, FREAC-1 [1].
• The part of FREAC-2 that binds TBP was mapped to 21 amino acids in the C-terminal end of the forkhead domain [1].
• TFIIB binds FREAC-2 close to the cleft between its two globular domains [1].
• Mutagenesis of the FOXF1/FOXF2 site reduced basal promoter activity by 50-60% and markedly attenuated transactivation of the promoter by FOXF1 [2].
• The gene for the human transcription factor forkhead related activator 2 (FREAC-2; HGMW-approved symbol FKHL6) has been characterized and found to consist of two exons separated by an intron of 3.6 kb [3].

Biological context of FOXF2

• DNase I footprint analysis of the fragment revealed a protected region at nt -82/-77, which is in a putative FOXF1/FOXF2 binding site [2].

Other interactions of FOXF2

• The target site for FREAC-2 on TBP was localized to the N-terminal repeat in the core domain of TBP [1].
• FREAC-2 was shown to interact in vitro with TBP and TFIIB [1].

Analytical, diagnostic and therapeutic context of FOXF2

• Fluorescence in situ hybridization was used to localize the human FREAC-2 gene to chromosomal position 6p24-p25, and the localization was further refined by radiation hybrid mapping to 6p25.3 [3].

References