High impact information on GNB2

• Restriction mapping and hybridization data position this gene (GNB2) to within 30-70 kilobases of the EPO gene [1].
• The selective isolation and mapping of GNB2 confirms the feasibility of this direct selection strategy and suggests that it will be useful for the rapid isolation of cDNAs, including disease-related genes, across extensive portions of the human genome [1].
• The beta subunits were also assigned--GNB1 to chromosome 1 and GNB2 to chromosome 7 [2].
• G protein beta2 subunit-derived peptides for inhibition and induction of G protein pathways. Examination of voltage-gated Ca2+ and G protein inwardly rectifying K+ channels [3].
• Ataxin 10 induces neuritogenesis via interaction with G-protein beta2 subunit [4].

Biological context of GNB2

• By using yeast-two-hybrid screening of a human brain cDNA library, we identified the G-protein beta2 subunit (Gbeta2) as an ataxin 10 binding partner, and the interaction was confirmed by coimmunoprecipitation and colocalization in mammalian cells in culture [4].
• G protein beta2 subunit antisense oligonucleotides inhibit cell proliferation and disorganize microtubule and mitotic spindle organization [5].

Other interactions of GNB2

• Whereas 3 genes (GNB2, EPO, PCOLCE) were known previously on the mRNA level, we have been able to identify 10 novel genes using a newly developed automatic annotation tool RUMMAGE-DP, which comprises >26 different programs mainly for exon prediction, homology searches, and compositional and repeat analysis [6].

References