Disease relevance of pcaU

• Native PcaU was purified after overexpression of the pcaU gene in Escherichia coli [1].
• Two different binding sites for Pseudomonas putida PcaR differ from the consensus in only a single nucleotide residue, and DNA directly downstream from Acinetobacter pcaU contains a 19-bp segment differing from the consensus in only two residues [2].

High impact information on pcaU

• The increase of the pcaU gene copy number leads to a decrease of the basal expression at pcaIp, indicating that the occupancy of the PcaU binding site is well balanced and depends on the concentration of PcaU in the cell [3].
• A PcaU binding site downstream of pcaU is covered by PcaU across two palindromic sequence repetitions [1].
• Expression at the pcaU gene promoter is independent of the growth condition but is subject to strong negative autoregulation [4].
• Downstream from pcaU are two open reading frames encoding proteins similar in amino acid sequence to those associated with the oxidation of acyl thioesters [2].
• Inactivation of pcaU reduced the induced expression of pca structural genes by about 90% and impeded but did not completely prevent growth of the mutant cells with protocatechuate [2].

References