High impact information on EB1

• Dictyostelium DdCP224 is a microtubule-associated protein and a permanent centrosomal resident involved in centrosome duplication [1].
• This effect was (i) assessed by turbidity measurements, pelleting of microtubules, and electron microscopy, (ii) observed when tubulin assembly was induced by taxol as well as by GTP in the presence of microtubule-associated proteins or glutamate, and (iii) specific as it was not produced by the phosphofructokinase from rabbit muscle [2].
• Among these mutants, some should contain electrophoretically altered tubulin, microtubule-associated proteins, or components of the amoebal cytoskeleton [3].

Anatomical context of EB1

• When soluble microtubule protein was fractionated into tubulin and microtubule-associated proteins and each fraction was assayed independently for its susceptibility toward inhibition, it was clearly demonstrated that the tubulin but not the associated protein fraction was rendered nonpolymerizable [4].

Analytical, diagnostic and therapeutic context of EB1

• To examine the role of cytoskeletal components during ameboid locomotion, immunofluorescence microscopy with antibodies to actin, myosin, and to a microtubule-associated component was used [5].

References