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Gene Review:

mTTF - mitochondrial transcription termination...

Drosophila melanogaster

Synonyms: BG:DS01068.4, CG18124, DS01068.4, DmTTF, Dmel\CG18124

Roberti, M. et al., Roberti, M. et al., Roberti, M. et al.

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Disease relevance of mTTF

In order to test whether this protein acts as transcription termination factor, we assayed the capacity of DmTTF to arrest in vitro the transcription catalyzed by mitochondrial and bacteriophage RNA polymerases [1].

High impact information on mTTF

In addition, the reported data provide interesting insights into the involvement of DmTTF in transcription initiation in Drosophila mitochondria [2].
To obtain in vivo evidences on the role of DmTTF, we characterized a DmTTF knock-down phenotype obtained by means of RNA interference in D.Mel-2 cells [2].
Band-shift and DNase I protection experiments show that DmTTF binds two homologous, short, non-coding sequences of Drosophila mitochondrial DNA, located at the 3' end of blocks of genes transcribed on opposite strands [3].
Using a combination of bioinformatic and molecular biology approaches a Drosophila melanogaster protein, DmTTF, has been identified, which exhibits sequence and structural similarity with two mitochondrial transcription termination factors, mTERF (human) and mtDBP (sea urchin) [3].
The existence of two DmTTF binding sites might serve not only to stop transcription but also to control the overlapping of a large number of transcripts generated by the peculiar transcription mechanism operating in this organism [3].

References

In vitro transcription termination activity of the Drosophila mitochondrial DNA-binding protein DmTTF. Roberti, M., Fernandez-Silva, P., Polosa, P.L., Fernandez-Vizarra, E., Bruni, F., Deceglie, S., Montoya, J., Gadaleta, M.N., Cantatore, P. Biochem. Biophys. Res. Commun. (2005) [Pubmed]
The Drosophila termination factor DmTTF regulates in vivo mitochondrial transcription. Roberti, M., Bruni, F., Polosa, P.L., Gadaleta, M.N., Cantatore, P. Nucleic Acids Res. (2006) [Pubmed]
DmTTF, a novel mitochondrial transcription termination factor that recognises two sequences of Drosophila melanogaster mitochondrial DNA. Roberti, M., Polosa, P.L., Bruni, F., Musicco, C., Gadaleta, M.N., Cantatore, P. Nucleic Acids Res. (2003) [Pubmed]

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