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Gene Review

pWBG738_03  -  inducible macrolide, lincosamide and...

Staphylococcus aureus

 
 
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Disease relevance of pWBG738_03

  • A 50S ribosomal subunit precursor particle is a substrate for the ErmC methyltransferase in Staphylococcus aureus cells [1].
 

High impact information on pWBG738_03

  • It was postulated that this sequence duplication affects the possible RNA conformations so that the ribosome binding site for ErmC synthesis is readily accessible to the ribosomes and thus constitutive expression of the ermC gene occurs [2].
  • Southern blot hybridisation with gene probes specific for staphylococcal erm genes demonstrated that the macrolide resistance gene belonged to hybridisation class C. Analysis of the ermC gene revealed that the deduced amino-acid sequence of the pSES21-encoded ErmC methylase exhibited c. 93% identity with the ErmC methylase encoded by plasmid pE194 [3].
  • High throughput chemical file screening with an enzymatic assay to detect inhibitors of the ErmC methyltransferase enzyme from macrolide-lincosamide-streptogramin B (MLSB) resistant pathogenic bacteria identified low molecular weight compounds that had IC50S (50% inhibitory concentration) in the nMolar to microMolar range [4].
  • Selective inhibitors of the ErmC methyltransferase were tested in tertiary assays to determine their minimal inhibitory concentrations (MICs), as single agents and in combination with the macrolide, azithromycin, against strains of pathogenic bacteria expressing MLSB-resistance [4].

References

  1. A 50S ribosomal subunit precursor particle is a substrate for the ErmC methyltransferase in Staphylococcus aureus cells. Champney, W.S., Chittum, H.S., Tober, C.L. Curr. Microbiol. (2003) [Pubmed]
  2. Tandem duplication in ermC translational attenuator of the macrolide-lincosamide-streptogramin B resistance plasmid pSES6 from Staphylococcus equorum. Lodder, G., Schwarz, S., Gregory, P., Dyke, K. Antimicrob. Agents Chemother. (1996) [Pubmed]
  3. Molecular analysis of the macrolide-lincosamide resistance gene region of a novel plasmid from Staphylococcus hyicus. Schwarz, S., Lange, C., Werckenthin, C. J. Med. Microbiol. (1998) [Pubmed]
  4. Assays to detect and characterize synthetic agents that inhibit the ErmC methyltransferase. Clancy, J., Schmieder, B.J., Petitpas, J.W., Manousos, M., Williams, J.A., Faiella, J.A., Girard, A.E., McGuirk, P.R. J. Antibiot. (1995) [Pubmed]
 
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