Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Editor

Personal info

View

About

Terms

Javascript disabled

Please enable Javascript support in your browser to use this application.

Instructions on how to enable JavaScript on different browsers can be found here: http://www.google.com/support/bin/answer.py?answer=23852.

[edit this page]

Gene Review:

fabI - enoyl-(acyl carrier protein) reductase

Staphylococcus aureus RF122

Ji, Y. et al., Yin, D. et al., Marrakchi, H. et al., Brenwald, N.P. et al.

Brenwald, Fraise,

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of fabI

Antisense isogenic strains expressing antisense RNA to fabI were created using a tetracycline-regulated vector in Staphylococcus aureus [1].

High impact information on fabI

However, the genes did not complement a fabI (Ts) mutant and the purified flavoproteins were unable to reduce enoyl-ACP in vitro and did not exhibit NAD(P)H oxidase activity, indicating they were not enoyl-ACP reductases [2].
We demonstrated that down-regulation of FabI expression by induction of fabI antisense RNA induces a conditional lethal phenotype [1].
The lack of fabI mutations in one mutant and three of the clinical isolates showing reduced triclosan susceptibility suggest that genetic loci other than fabI may be involved in triclosan resistance [3].
In contrast, strains expressing antisense to isoleucine tRNA synthetase (ileS) and enoyl-[acyl-carrier-protein] reductase (fabI) were specifically identified as having increased susceptibility to mupirocin and triclosan, respectively [4].

References

Validation of antibacterial mechanism of action using regulated antisense RNA expression in Staphylococcus aureus. Ji, Y., Yin, D., Fox, B., Holmes, D.J., Payne, D., Rosenberg, M. FEMS Microbiol. Lett. (2004) [Pubmed]
Characterization of Streptococcus pneumoniae enoyl-(acyl-carrier protein) reductase (FabK). Marrakchi, H., Dewolf, W.E., Quinn, C., West, J., Polizzi, B.J., So, C.Y., Holmes, D.J., Reed, S.L., Heath, R.J., Payne, D.J., Rock, C.O., Wallis, N.G. Biochem. J. (2003) [Pubmed]
Triclosan resistance in methicillin-resistant Staphylococcus aureus (MRSA). Brenwald, N.P., Fraise, A.P. J. Hosp. Infect. (2003) [Pubmed]
Identification of antimicrobial targets using a comprehensive genomic approach. Yin, D., Fox, B., Lonetto, M.L., Etherton, M.R., Payne, D.J., Holmes, D.J., Rosenberg, M., Ji, Y. Pharmacogenomics (2004) [Pubmed]

Contributions to this collaborative article are from individual authors of WikiGenes or mined by the WikiGenes Data Mining Engine from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.About WikiGenes Open Access Licence Privacy Policy Terms of Use apsburg

The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.