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Gene Review: 

ENAM  -  enamelin

Sus scrofa

 
 
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High impact information on ENAM

  • Searching using the amino acid sequence determined in this study showed it to be also present in the 32 kDa and 89 kDa enamelin proteins reported by Fukae, Tanabe, Murakami and Tohi [(1996) Adv. Dent. Res., in the press] [1].
  • Their enamelin characteristic was confirmed by hydroxyapatite-binding studies and amino acid analysis [2].
  • Immunocytochemical and immunochemical study of enamelins, using antibodies against porcine 89-kDa enamelin and its N-terminal synthetic peptide, in porcine tooth germs [3].
  • The structures of asparagine-linked oligosaccharides of porcine 32 kDa enamelin are reported [4].
  • Dissociative extraction with urea subsequently extracted the remainder of the enamelin-processing products present [5].
 

Biological context of ENAM

  • In this study, glycopeptides containing all three potential glycosylation sites (72-Asn, 79-Asn and 91-Asn) were purified from 32 kDa enamelin [6].

References

  1. Identification of a novel proteinase (ameloprotease-I) responsible for the complete degradation of amelogenin during enamel maturation. Moradian-Oldak, J., Leung, W., Simmer, J.P., Zeichner-David, M., Fincham, A.G. Biochem. J. (1996) [Pubmed]
  2. Isolation and characterization of pig enamelins. Limeback, H. Biochem. J. (1987) [Pubmed]
  3. Immunocytochemical and immunochemical study of enamelins, using antibodies against porcine 89-kDa enamelin and its N-terminal synthetic peptide, in porcine tooth germs. Dohi, N., Murakami, C., Tanabe, T., Yamakoshi, Y., Fukae, M., Yamamoto, Y., Wakida, K., Shimizu, M., Simmer, J.P., Kurihara, H., Uchida, T. Cell Tissue Res. (1998) [Pubmed]
  4. Carbohydrate moieties of porcine 32 kDa enamelin. Yamakoshi, Y. Calcif. Tissue Int. (1995) [Pubmed]
  5. Enamelin compartmentalization in developing porcine enamel. Brookes, S.J., Lyngstadaas, S.P., Robinson, C., Shore, R.C., Wood, S.R., Kirkham, J. Connect. Tissue Res. (2002) [Pubmed]
  6. Sites of asparagine-linked oligosaccharides in porcine 32 kDa enamelin. Yamakoshi, Y., Pinheiro, F.H., Tanabe, T., Fukae, M., Shimizu, M. Connect. Tissue Res. (1998) [Pubmed]
 
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