Disease relevance of TKT

• Three calves were infected with the parental strain BHV-1 w/t, three with the TK-defective strain, B 1 and four with the HPMPA-resistant strain, 3 A. Inoculation with w/t virus resulted in a reproducible clinical disease characterised by respiratory distress, fever and the presence of virus in nasal mucus [1].
• The thiamin status (thiamin concentration in whole blood, plasma, and erythrocytes; erythrocyte transketolase activity) of normal cattle consuming varying diets did not differ from that of cattle with polioencephalomalacia or lead poisoning [2].

High impact information on TKT

• The affected animals did not respond to vitamin B1 treatment; the erythrocyte transketolase levels of in-contact cattle and of one untreated affected calf and one untreated lamb were within the normal range [3].
• Antibody formation after treatment was markedly different in wild type and in TK- virus inoculated groups [4].
• In the former, virus reactivation was suggested by a sudden rise in serum antibody levels with kinetics closely resembling those reported in infected calves following corticosteroid administration, whereas in the case of the TK- group no significant increase in antibody activity was measured [4].
• The transketolase activity in beef liver enzyme preparation was extremely inhibited by PRPP and ADP, but the transaldolase activity was not inhibited [5].
• Transketolase activity in the blood of cattle and sheep in relation to thiamine deficiency [6].

Anatomical context of TKT

• The thiamine concentration of liver, brain and heart was determined and erythrocyte transketolase assessed [7].

Associations of TKT with chemical compounds

• The thiamine pyrophosphate effect on erythrocyte transketolase activity was unaltered in calves of either diet group [8].

Analytical, diagnostic and therapeutic context of TKT

• Significant decrease in a thiamine-dependent enzyme, erythrocyte transketolase activity, and increase in thiamine pyrophosphate effect were detected about 2 weeks before the onset of abnormal electroencephalograms [9].

References