High impact information on PRH1

• Alleles at the PRH1 locus coding for the human salivary-acidic proline-rich proteins Pa, Db, and PIF [1].
• The 4-kb region of sequenced DNA from PRH1 differs by an average of 8.7% from the same region in PRH2, but the nucleotide sequences of the exon 3 of the two genes differ by only 0.2% [2].
• Two sets of repeats occur in the sequenced region in addition to the 63-bp repeats: one pair of about 140 bp flanks 500 bp of DNA in the first intervening sequence, and the other pair of 72 bp is tandemly repeated 1.4 kb 5' to the PRH1 gene [2].
• In order to determine salivary PRH1 phenotypes it is necessary to use SDS or basic gel electrophoresis in addition to the isoelectric focusing gel electrophoresis [3].
• The allele frequencies of the PRH1 locus in Japanese were PRH1 (double-band protein) = 0.035, PRH1(2) (acidic protein) = 0.193, PRH1(4) (PIf) = 0.751, and PRH1(5) (As) = 0.021 [3].

Biological context of PRH1

• The data obtained permit the identification of PRP polymorphisms and phenotypes, the determination of the relative amounts of PRPs derived from the two loci, PRH1 and PRH2, and the measurement of the extent of post-translational cleavage of the primary polypeptide products [4].

Anatomical context of PRH1

• Nailfold skin capillary blood cell velocity (CBV) was studied at rest and during post-occlusive reactive hyperaemia after a 1 min arterial occlusion (PRH1) in: (i) both hands of patients with arterial obliterative disease (AOD) of one arm, (ii) patients with polycythaemia, before and after a moderate haemodilution and (iii) healthy controls [5].

Associations of PRH1 with chemical compounds

• A biotin-labelling in situ hybridization technique was first used to physically map two single copy genes, cdc2 and prh1, in maize [6].

Other interactions of PRH1

• Genes at two of these loci (proposed names, PRH1 and PRH2) contain regions that strongly hybridize to a probe made from a cDNA in which sites for the restriction enzyme HaeIII occur repeatedly; they code for the acidic PRPs [7].
• In order to assess the possible occurrence of acute haemolysis with prolonged exertion, serum haptoglobin levels were determined from venous blood samples collected from eight male runners immediately preceding (PreRH), immediately following (PRH1), and 6 h following (PRH2) completion of a marathon road race [8].

References