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MeSH Review

Isoelectric Focusing

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Disease relevance of Isoelectric Focusing


Psychiatry related information on Isoelectric Focusing

  • The possibility that the different molecular forms of acetylcholinesterase (AChE) in cerebrospinal fluid (CSF) which can be revealed by isoelectric focusing may reflect changes in AChE in pathologically affected neurons in Alzheimer's disease was tested in a retrospective study [6].
  • Isoelectric focusing (IEF) analysis of affinity-purified Tf, under conditions of total iron saturation, identifies a major isoform at pI 5.4 in both normal consumers and alcohol abusers; three additional Tf isoforms (pI 5.6, 5.7, and 5.8) are associated with alcohol abuse [7].
  • Serum samples of 37 AD and 13 Alcohol-induced dementia patients as well as 10 healthy controls were analyzed for abnormal Tf variants, using isoelectric focusing followed by blotting with anti-Tf antibodies [8].

High impact information on Isoelectric Focusing


Chemical compound and disease context of Isoelectric Focusing


Biological context of Isoelectric Focusing


Anatomical context of Isoelectric Focusing


Associations of Isoelectric Focusing with chemical compounds

  • Isoelectric focusing in agarose, and two-dimensional polyacrylamide gel electrophoresis revealed that the antigens had pI between 4.5 and 6.5, but that the 17/19 kD antigens were more acidic than the 38 kD antigen [29].
  • This component was noted to be heterodisperse both by size on SDS gels and by charge on isoelectric focusing gels [30].
  • Isoelectric focusing shows that the alpha subunits (pI 4.4-4.7) are more acidic than the beta subunits (pI 6.0-6.2) but that each glycoprotein species is distinctive [31].
  • Three forms of C2 have been identified by isoelectric focusing in polyacrylamide gel and development of patterns in agarose gel containing antibody-sensitized sheep red cells and C2-deficient serum: C2 C (common), C2 A (acidic), and C2 B (basic) [32].
  • Antibodies to hapten 2-phenyloxazolone (phOx) of all BALB/c and DBA/2 mice have the same idiotype and the same major (public) isoelectric focusing pattern whose main spectrotype is called Ox-1 [33].

Gene context of Isoelectric Focusing


Analytical, diagnostic and therapeutic context of Isoelectric Focusing


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