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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

POLR3D  -  polymerase (RNA) III (DNA directed)...

Homo sapiens

Synonyms: BN51, BN51T, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, Protein BN51, ...
 
 
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High impact information on POLR3D

  • The human BN51 gene was originally isolated as a suppressor of a temperature-sensitive cell cycle mutant of BHK cells (tsBN51) [1].
  • The BN51 cDNA was originally isolated by its ability to complement a temperature-sensitive hamster cell mutant that undergoes a G1 cell division arrest, as is true for the rpc53 mutants [2].
  • When the yeast gene is introduced into the BN51 cells in a mammalian expression vector, it weakly complements the BN51 mutation in that it prevents cell death at 39.5 degrees C. The mechanism by which inhibition of RNA polymerase III activity leads to arrest in G1 is unclear but is not due to a marked decrease in total protein synthesis [3].
  • We have recently found that the predicted BN51 protein shows significant homology to the 53 kilodalton subunit of RNA polymerase C (III) from Saccharomyces cerevisiae [3].
  • Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis [4].
 

Analytical, diagnostic and therapeutic context of POLR3D

  • Consistent with this, antibodies raised to fusion proteins containing BN51 coding sequences and antipeptide antibodies reveal that the BN51 gene encodes a 48 kilodalton protein which appears to be located primarily in the nucleus following subcellular fractionation and by immunohistochemistry [3].

References

  1. The BN51 protein is a polymerase (Pol)-specific subunit of RNA Pol III which reveals a link between Pol III transcription and pre-rRNA processing. Jackson, A.J., Ittmann, M., Pugh, B.F. Mol. Cell. Biol. (1995) [Pubmed]
  2. RPC53 encodes a subunit of Saccharomyces cerevisiae RNA polymerase C (III) whose inactivation leads to a predominantly G1 arrest. Mann, C., Micouin, J.Y., Chiannilkulchai, N., Treich, I., Buhler, J.M., Sentenac, A. Mol. Cell. Biol. (1992) [Pubmed]
  3. The gene complementing a temperature-sensitive cell cycle mutant of BHK cells is the human homologue of the yeast RPC53 gene, which encodes a subunit of RNA polymerase C (III). Ittmann, M., Ali, J., Greco, A., Basilico, C. Cell Growth Differ. (1993) [Pubmed]
  4. Myc induces the nucleolin and BN51 genes: possible implications in ribosome biogenesis. Greasley, P.J., Bonnard, C., Amati, B. Nucleic Acids Res. (2000) [Pubmed]
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