Disease relevance of KIF18A

• A unique gene having homology with the kinesin family member 18A encodes a tumour-associated antigen recognised by cytotoxic T lymphocytes from HLA-A2+ colon cancer patients [1].

High impact information on KIF18A

• MS-KIF18A was cloned from a marrow stromal cells expression library [2].
• Examination of a three-dimensional model of the MS-KIF18A motor domain suggested that this protein associates with microtubules, which was confirmed by immunofluorescence (IF) experiments in stromal cells [2].
• We report here a unique gene, 3362 base-pairs (bp) long, which has homology with the kinesin family member 18A [1].
• Bioinformatics analysis suggested interactions between MS-KIF18A and estrogen receptor (ERalpha) which were further elucidated by immunoprecipitation (IP) [3].
• The activation was interfered when cells were pre-treated with either ICI-182,780 or MAPK inhibitor PD98059 prior the challenge with 17beta-E2 that resulted in a decrease in association between MS-KIF18A and p-ERK1/2 [3].

Anatomical context of KIF18A

• Moreover, MS-KIF18A and 66 kDa ERalpha complex has been demonstrated between ectopically expressed proteins in COS-7 cells [3].

Associations of KIF18A with chemical compounds

• This study presents a novel interaction between MS-KIF18A and ER that may have important physiological and pharmacological implications for estrogen action in various cells [3].

Analytical, diagnostic and therapeutic context of KIF18A

• Indirect immunofluorescence (IF) analyzed the intracellular distribution of endogenous MS-KIF18A and the transfected enhanced green fluorescence protein (eGFP)-MS-KIF18A in osteogenic cells [3].

References