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Gene Review

ZEO1  -  Zeo1p

Saccharomyces cerevisiae S288c

Synonyms: O0738, Protein ZEO1, YOL109W, Zeocin resistance protein 1
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High impact information on ZEO1

  • Our approach involves the random integration of a vector containing the Zeocin resistance gene into the yeast genome by restriction enzyme-mediated integration [1].
  • To further explore Mid2p signalling a two-hybrid screen was done using the cytoplasmic tail of Mid2p; this identified ZEO1 (YOL109w), encoding a 12 kDa peripheral membrane protein that localizes to the plasma membrane [2].
  • Marker recycling did not lead to increased zeocin resistance, indicating that the zeocin marker could be used for more than four rounds of transformation [3].
  • Zeocin resistance as a dominant selective marker for transformation and targeted gene deletions in Candida glabrata [4].
  • A Zeocin resistance gene on the plasmid is used to select high copy number tandem integrations of the plasmid constructs [5].


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