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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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Gene Review: 

ZEO1  -  Zeo1p

Saccharomyces cerevisiae S288c

Synonyms: O0738, Protein ZEO1, YOL109W, Zeocin resistance protein 1
 
 
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High impact information on ZEO1

  • Our approach involves the random integration of a vector containing the Zeocin resistance gene into the yeast genome by restriction enzyme-mediated integration [1].
  • To further explore Mid2p signalling a two-hybrid screen was done using the cytoplasmic tail of Mid2p; this identified ZEO1 (YOL109w), encoding a 12 kDa peripheral membrane protein that localizes to the plasma membrane [2].
  • Marker recycling did not lead to increased zeocin resistance, indicating that the zeocin marker could be used for more than four rounds of transformation [3].
  • Zeocin resistance as a dominant selective marker for transformation and targeted gene deletions in Candida glabrata [4].
  • A Zeocin resistance gene on the plasmid is used to select high copy number tandem integrations of the plasmid constructs [5].

References

  1. GSA11 encodes a unique 208-kDa protein required for pexophagy and autophagy in Pichia pastoris. Strømhaug, P.E., Bevan, A., Dunn, W.A. J. Biol. Chem. (2001) [Pubmed]
  2. A synthetic analysis of the Saccharomyces cerevisiae stress sensor Mid2p, and identification of a Mid2p-interacting protein, Zeo1p, that modulates the PKC1-MPK1 cell integrity pathway. Green, R., Lesage, G., Sdicu, A.M., Ménard, P., Bussey, H. Microbiology (Reading, Engl.) (2003) [Pubmed]
  3. Overproduction of pentose phosphate pathway enzymes using a new CRE-loxP expression vector for repeated genomic integration in Saccharomyces cerevisiae. Johansson, B., Hahn-Hägerdal, B. Yeast (2002) [Pubmed]
  4. Zeocin resistance as a dominant selective marker for transformation and targeted gene deletions in Candida glabrata. Alderton, A.J., Burr, I., M??hlschlegel, F.A., Tuite, M.F. Mycoses (2006) [Pubmed]
  5. Purification and assay of recombinant ADAR proteins expressed in the yeast Pichia pastoris or in Escherichia coli. Ring, G.M., O'Connell, M.A., Keegan, L.P. Methods Mol. Biol. (2004) [Pubmed]
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