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Gene Review:

RPA49 - DNA-directed RNA polymerase I subunit RPA49

Saccharomyces cerevisiae S288c

Synonyms: A49, DNA-directed RNA polymerase I 49 kDa polypeptide, N0880, RRN13, YNL248C

Iborra, F. et al., Gadal, O. et al., Martínez-Argudo, I. et al., Liljelund, P. et al., Riva, M. et al.

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Disease relevance of RPA49

Mutations A49P and Delta47-53 at the T loop of the Escherichia coli GlnB (PII) protein impair regulatory interactions with the two-component sensor regulator NtrB (P. Jiang, P. Zucker, M. R. Atkinson, E. S. Kamberov, W. Tirasophon, P. Chandran, B. R. Schepke, and A. J. Ninfa, J. Bacteriol. 179: 4342-4353, 1997) [1].

High impact information on RPA49

Conversely, rpa34-Delta mutants lacking Rpa34, a non-essential subunit associated with Rpa49, are lethal in top1-Delta but not in top3-Delta [2].
Hmo1 overexpression suppresses rpa49-Delta mutants lacking Rpa49, a non-essential but conserved subunit of RNA polymerase I corresponding to the animal RNA polymerase I factor PAF53 [2].
Insertion of the HIS3 gene halfway into the RPA49 coding region results in synthesis of a truncated A49 subunit that is incorporated into the polymerase [3].
Characterization and mutagenesis of the gene encoding the A49 subunit of RNA polymerase A in Saccharomyces cerevisiae [3].
One meiotic segregant had a hybrid RNA polymerase A with five of the polymorphic polypeptides (A49, A43, A19, A14.5, and A14) coming from S. douglasii and two (A40 and A34.5) from S. cerevisiae [4].

Associations of RPA49 with chemical compounds

They co-migrate on sodium dodecyl sulfate-gel electrophoresis, have the same chromatographic properties, and dissociate simultaneously from RNA polymerase A. Fractions containing RNase H49 stimulate RNA synthesis by RNA polymerase A* lacking A49 and A34.5 subunits [5].

Co-localisations of RPA49

Two ribonuclease H activities have been found in yeast RNA polymerase A. The nuclease activities comigrated with subunits A49 (Mr = 49,000) and A40 (Mr = 40,000), after electrophoresis in a sodium dodecyl sulfate polyacrylamide gel containing [32P](rG)n . (dC)n as substrate [5].

Other interactions of RPA49

These data identify Hmo1 as a genuine RNA polymerase I factor acting synergistically with Rpa49 [2].
Several lines of evidence suggest that the chromatin RNase H of 49,000 daltons (RNase H49) is the same protein as subunit A49 [5].

References

PII T-loop mutations affecting signal transduction to NtrB also abolish yeast two-hybrid interactions. Martínez-Argudo, I., Contreras, A. J. Bacteriol. (2002) [Pubmed]
Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system. Gadal, O., Labarre, S., Boschiero, C., Thuriaux, P. EMBO J. (2002) [Pubmed]
Characterization and mutagenesis of the gene encoding the A49 subunit of RNA polymerase A in Saccharomyces cerevisiae. Liljelund, P., Mariotte, S., Buhler, J.M., Sentenac, A. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
Natural variation in yeast RNA polymerase A. Formation of a mosaic RNA polymerase A in a meiotic segregant from an interspecific hybrid. Riva, M., Buhler, J.M., Sentenac, A., Fromageot, P., Hawthorne, D.C. J. Biol. Chem. (1982) [Pubmed]
Identification of two different RNase H activities associated with yeast RNA polymerase A. Iborra, F., Huet, J., Breant, B., Sentenac, A., Fromageot, P. J. Biol. Chem. (1979) [Pubmed]

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AGOS_AFR638C	Ashbya gossypii ATCC 10895
KLLA0C02959g	Kluyveromyces lactis NRRL Y-1140
rpa49	Schizosaccharomyces pombe 972h-

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