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Gene Review

HMO1  -  Hmo1p

Saccharomyces cerevisiae S288c

Synonyms: HSM2, High mobility group protein 1, High spontaneous mutagenesis protein 2, YD9395.07, YDR174W
 
 
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High impact information on HMO1

  • Hmo1 overexpression suppresses rpa49-Delta mutants lacking Rpa49, a non-essential but conserved subunit of RNA polymerase I corresponding to the animal RNA polymerase I factor PAF53 [1].
  • Hmo1 co-localizes with Fob1, a known rDNA-binding protein, defining a narrow territory adjacent to the nucleoplasm that could delineate the rDNA nucleolar domain [1].
  • Among them is Abf2p, an abundant, high-mobility group protein that is known to function in nucleoid morphology, and in mtDNA transactions [2].
  • A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria [3].
  • Lastly, like mammalian UBF, Hmo1 associates at many locations throughout the rRNA gene locus, and it is important for processing of rRNA in addition to its role in rRNA transcription [4].
 

Biological context of HMO1

  • Taken together, the data indicate that HMO1 is likely to be the homolog of HMG1/2 in higher cells and that it plays an important role in genome maintenance [5].
  • HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae [6].
  • HMO1 bends DNA, as measured by DNA cyclization assays, facilitating cyclization of 136-, 105-, and 87-bp DNA, but not 75-bp DNA, and it has a significantly longer residence time on DNA minicircles compared with linear duplex DNA [7].
  • Determination of DNA substrate specificity using electrophoretic mobility shift assays shows unexpectedly that the lysine-rich tail does not increase DNA binding affinity but instead is required for DNA bending by full-length HMO1; HMO1-boxBC, lacking the box A domain, also fails to bend DNA [8].
  • Amino acid sequence alignment suggests that this protein, Hmo1p, is related to the HMG1/2 class of chromatin-associated proteins [5].
 

Associations of HMO1 with chemical compounds

  • As HMO1-boxAB, lacking only the lysine-rich C-terminal segment, exhibits two melting transitions at 46.0 and 63.3 degrees C, we conclude that the destabilization of the box A domain seen in full-length HMO1 is due primarily to its interaction with the lysine-rich tail [8].
  • Determinants of DNA binding and bending by the Saccharomyces cerevisiae high mobility group protein NHP6A that are important for its biological activities. Role of the unique N terminus and putative intercalating methionine [9].
 

Other interactions of HMO1

  • We find that mutations in HMO1, which encodes a high mobility group 1/2 homolog, are synthetically lethal with mutations in the yeast FPR1 gene encoding FKBP12 [10].
  • The frequency of UV-induced mutations in hsm2 rev3 was not altered in comparison with single rev3 mutant [6].
  • The UV-induced mutation frequency in double hsm2 hsm3 mutant was lower than in the single hsm2 and hsm3 mutants [6].
  • These data identify Hmo1 as a genuine RNA polymerase I factor acting synergistically with Rpa49 [1].
 

Analytical, diagnostic and therapeutic context of HMO1

References

  1. Hmo1, an HMG-box protein, belongs to the yeast ribosomal DNA transcription system. Gadal, O., Labarre, S., Boschiero, C., Thuriaux, P. EMBO J. (2002) [Pubmed]
  2. In organello formaldehyde crosslinking of proteins to mtDNA: identification of bifunctional proteins. Kaufman, B.A., Newman, S.M., Hallberg, R.L., Slaughter, C.A., Perlman, P.S., Butow, R.A. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
  3. A close relative of the nuclear, chromosomal high-mobility group protein HMG1 in yeast mitochondria. Diffley, J.F., Stillman, B. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  4. An HMG protein, Hmo1, associates with promoters of many ribosomal protein genes and throughout the rRNA gene locus in Saccharomyces cerevisiae. Hall, D.B., Wade, J.T., Struhl, K. Mol. Cell. Biol. (2006) [Pubmed]
  5. Characterization of a high mobility group 1/2 homolog in yeast. Lu, J., Kobayashi, R., Brill, S.J. J. Biol. Chem. (1996) [Pubmed]
  6. HSM2 (HMO1) gene participates in mutagenesis control in yeast Saccharomyces cerevisiae. Alekseev, S.Y., Kovaltsova, S.V., Fedorova, I.V., Gracheva, L.M., Evstukhina, T.A., Peshekhonov, V.T., Korolev, V.G. DNA Repair (Amst.) (2002) [Pubmed]
  7. The Saccharomyces cerevisiae high mobility group box protein HMO1 contains two functional DNA binding domains. Kamau, E., Bauerle, K.T., Grove, A. J. Biol. Chem. (2004) [Pubmed]
  8. Interactions between N- and C-terminal domains of the Saccharomyces cerevisiae high-mobility group protein HMO1 are required for DNA bending. Bauerle, K.T., Kamau, E., Grove, A. Biochemistry (2006) [Pubmed]
  9. Determinants of DNA binding and bending by the Saccharomyces cerevisiae high mobility group protein NHP6A that are important for its biological activities. Role of the unique N terminus and putative intercalating methionine. Yen, Y.M., Wong, B., Johnson, R.C. J. Biol. Chem. (1998) [Pubmed]
  10. Hmo1p, a high mobility group 1/2 homolog, genetically and physically interacts with the yeast FKBP12 prolyl isomerase. Dolinski, K.J., Heitman, J. Genetics (1999) [Pubmed]
  11. A high mobility group protein binds to long CAG repeat tracts and establishes their chromatin organization in Saccharomyces cerevisiae. Kim, H., Livingston, D.M. J. Biol. Chem. (2006) [Pubmed]
 
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