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Gene Review:

URK1 - uridine kinase URK1

Saccharomyces cerevisiae S288c

Synonyms: N2050, Uridine kinase, Uridine monophosphokinase, YNR012W

Mitterbauer, R. et al., Petersen, C. et al., Mitterbauer, R. et al., Liljelund, P. et al.

Petersen, Møller,

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High impact information on URK1

This has been presumed to involve phosphorylation of cytidine to CMP by cytidine/uridine kinase and subsequent hydrolysis of CMP to cytosine and ribose 5-phosphate by a putative CMP hydrolase [1].
To prevent interference with pyrimidines potentially present in biological samples, we also disrupted the genes FUR1 and URK1, blocking the pyrimidine salvage pathway [2].
Disruption of this gene in a conditionally pyrimidine-auxotrophic S. cerevisiae strain, which is also deficient in uridine kinase (urk1), leads to the inability of the mutant to utilize uridine as the sole source of pyrimidines [3].
We selected a 5-fluorouracil-resistant, thermosensitive mutant of the uridine monophosphokinase step in Saccharomyces cerevisiae [4].

References

The RihA, RihB, and RihC ribonucleoside hydrolases of Escherichia coli. Substrate specificity, gene expression, and regulation. Petersen, C., Møller, L.B. J. Biol. Chem. (2001) [Pubmed]
A sensitive and inexpensive yeast bioassay for the mycotoxin zearalenone and other compounds with estrogenic activity. Mitterbauer, R., Weindorfer, H., Safaie, N., Krska, R., Lemmens, M., Ruckenbauer, P., Kuchler, K., Adam, G. Appl. Environ. Microbiol. (2003) [Pubmed]
Saccharomyces cerevisiae URH1 (encoding uridine-cytidine N-ribohydrolase): functional complementation by a nucleoside hydrolase from a protozoan parasite and by a mammalian uridine phosphorylase. Mitterbauer, R., Karl, T., Adam, G. Appl. Environ. Microbiol. (2002) [Pubmed]
Genetic characterization and isolation of the Saccharomyces cerevisiae gene coding for uridine monophosphokinase. Liljelund, P., Lacroute, F. Mol. Gen. Genet. (1986) [Pubmed]

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AgaP_AGAP005209	Anopheles gambiae str. PEST
UK/UPRT1	Arabidopsis thaliana
UKL2	Arabidopsis thaliana
AGOS_ADR288W	Ashbya gossypii ATCC 10895
F19B6.1	Caenorhabditis elegans
si:ch211-243j20.2	Danio rerio
l(2)k01209	Drosophila melanogaster
LOC776992	Gallus gallus
KLLA0F06182g	Kluyveromyces lactis NRRL Y-1140
MGG_12159	Magnaporthe oryzae 70-15
NCU05224	Neurospora crassa OR74A
Os08g0530000	Oryza sativa Japonica Group
Os09g0505800	Oryza sativa Japonica Group
SPCC162.11c	Schizosaccharomyces pombe 972h-
uckl1	Xenopus (Silurana) tropicalis

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