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Gene Review:

mbtB - phenyloxazoline synthase

Mycobacterium tuberculosis H37Rv

Quadri, L.E. et al., Gold, B. et al., De Voss, J.J. et al.

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Disease relevance of mbtB

Overproduction and purification of the mbtB ArCP domain and MbtA in Escherichia coli allowed validation of the mycobactin initiation hypothesis, as sequential action of PptT (a phosphopantetheinyl transferase) and MbtA (a salicyl-AMP ligase) resulted in the mbtB ArCP domain being activated as salicyl-S-ArCP [1].

High impact information on mbtB

We have used gene replacement through homologous recombination to delete the mbtB gene and replace this with a hygromycin-resistance cassette in the virulent strain of M. tuberculosis H37Rv [2].
We analysed four promoter regions containing putative IdeR boxes, mbtA-mbtB, mbI, rv3402c and bfrA-bfd, for interaction with IdeR and for iron-dependent expression [3].
Three of the IdeR- and iron-repressed genes, mbtB, mbtI and rv3402c, were induced during M. tuberculosis infection of human THP-1 macrophages [3].

References

Identification of a Mycobacterium tuberculosis gene cluster encoding the biosynthetic enzymes for assembly of the virulence-conferring siderophore mycobactin. Quadri, L.E., Sello, J., Keating, T.A., Weinreb, P.H., Walsh, C.T. Chem. Biol. (1998) [Pubmed]
The salicylate-derived mycobactin siderophores of Mycobacterium tuberculosis are essential for growth in macrophages. De Voss, J.J., Rutter, K., Schroeder, B.G., Su, H., Zhu, Y., Barry, C.E. Proc. Natl. Acad. Sci. U.S.A. (2000) [Pubmed]
The Mycobacterium tuberculosis IdeR is a dual functional regulator that controls transcription of genes involved in iron acquisition, iron storage and survival in macrophages. Gold, B., Rodriguez, G.M., Marras, S.A., Pentecost, M., Smith, I. Mol. Microbiol. (2001) [Pubmed]

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