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Chemical Compound Review

Homomycin     (E)-N-[(3aS,4R,5R,6S,7R,7aR)- 4,6,7...

Synonyms: Totomycin, Hygromycin A, CHEMBL267436, CHEBI:69414, AC1O5GAM, ...
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Disease relevance of HYGROMYCIN

  • B16F10 melanoma cells were stably transfected with alpha(1,3/1,4) fucosyltransferase-specific cDNA (B16F10ft), allowing them to express E-selectin ligands or with hygromycin resistance selection vector only B16F10hygro) [1].
  • To investigate the effect of a mutation in ums2, a plasmid was constructed in which most of the transcriptional unit of ums2 was deleted and substituted with the Escherichia coli hygromycin B (hygB) phosphotransferase gene [2].
  • A retroviral vector ribozyme library with randomized target recognition sequences was introduced into HeLa cells, stably expressing a bicistronic construct encoding the hygromycin B phosphotransferase gene and the herpes simplex virus thymidine kinase gene (HSV-tk) [3].
  • Because we incorporated the genes for hygromycin resistance and green fluorescent protein onto the E. coli cloned EBV genome, the still open question of the EBV target cells other than B lymphocytes will be addressed [4].
  • The stably transfected hygromycin-resistant colonies were screened for DT hypersensitivity employing a replica plate system [5].

High impact information on HYGROMYCIN

  • A Xenopus cell line, X-C, was stably transfected with plasmids containing a hygromycin-resistance gene and genes for either beta-galactosidase with a heat shock promoter or chloramphenicol acetyltransferase (CAT) with a muscle-specific actin promoter [6].
  • A hygromycin B resistance (hygro(R)) gene was inserted into the human beta-globin LCR on chromosome 11 in a mouse/human hybrid erythroid cell line that expresses the human beta-globin gene after the induction of differentiation [7].
  • The cDNA for p47-phox was inserted into an EBV-expression vector and stably transfected cell lines were obtained using hygromycin B selection [8].
  • However, its deletion increases the plasma membrane potential and confers sensitivity to cytotoxic cations, such as Na(+) and hygromycin B [9].
  • To investigate further the physiological role of TR in Leishmania, we attempted to create TR-knockout mutants by gene disruption in L. donovani and L. major strains using the selectable markers neomycin and hygromycin phosphotransferases [10].

Chemical compound and disease context of HYGROMYCIN


Biological context of HYGROMYCIN

  • We inserted genes or gene segments, that code for the bacterial chloramphenicol acetyltransferase, the bacterial gene conferring resistance against hygromycin, and the ORF E7 of the human papillomavirus type 18 into these vectors [16].
  • Plants with recombination events were identified by selecting for hygromycin resistance, a phenotype expressed upon recombination [17].
  • Transposition of a Ds element marked with a hygromycin-resistance gene was activated from four different locations in the Arabidopsis genome [18].
  • A promoterless aph(3')II (aminoglycoside phosphotransferase II) reporter gene was linked to the right end of the T-DNA and transformed into plants along with a plasmid replicon and a selectable hygromycin-resistance gene [19].
  • We mutagenized Arabidopsis thaliana plants carrying a silent transgene encoding hygromycin phosphotransferase (hpt) and therefore show a heritable hygromycin-sensitive phenotype [20].

Anatomical context of HYGROMYCIN


Associations of HYGROMYCIN with other chemical compounds


Gene context of HYGROMYCIN


Analytical, diagnostic and therapeutic context of HYGROMYCIN


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