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Gene Review

gyrB  -  DNA gyrase subunit B

Mycobacterium tuberculosis H37Rv

 
 
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Disease relevance of gyrB

  • Mutations in the gyrA and gyrB genes of fluoroquinolone-resistant Mycobacterium tuberculosis from TB patients in Thailand [1].
 

High impact information on gyrB

  • Their quinolone resistance-determining regions for gyrA and gyrB were amplified and sequenced to identify mutations in gyrase A or B [2].
  • In contrast, the subtype I strains as well as the M. africanum type strain showed unique combinations of SNPs in gyrB and genomic deletions (the absence of RD9 and the presence of RD12), which proves their independence from M. tuberculosis and M. bovis [3].
  • The present investigation evaluated the PCR-restriction fragment length polymorphism (RFLP) analysis of hsp65 and gyrB targets for differentiation of the species within the Mycobacterium tuberculosis complex (MTC) both by including new restriction enzymes and previously unstudied species [4].
  • From DNA-sequencing, 21 of 35 (60%) exhibited single-point mutations in different positions, at Ala90Val, Ser91Pro, and Asp94(Gly/Ala/His/Asn); and one novel mutation position at Gly88Cys in the gyrA gene and Asp495Asn in the gyrB gene [1].
  • Mutation screening was done by amplifying the pncA, gyrA, and gyrB genes by the polymerase chain reaction (PCR) and direct automated sequencing [5].
 

Biological context of gyrB

 

Analytical, diagnostic and therapeutic context of gyrB

  • In this study, 35 isolates of FQr-MTB were collected from Siriraj Hospital and Chest Disease Institute. The quinolone-resistance-determining regions (QRDR) of gyrA and gyrB genes in all 35 FQr-MTB isolates and from the H37Ra MTB strain were amplified using polymerase chain reaction (PCR) [1].
  • A study of the single nucleotide polymorphisms (SNP) within the gyrB by sequence analysis not only confirmed the results of the restriction analysis, but showed further differences among 'M. canettii' isolates that were not picked up using the existing battery of restriction enzymes [4].

References

  1. Mutations in the gyrA and gyrB genes of fluoroquinolone-resistant Mycobacterium tuberculosis from TB patients in Thailand. Pitaksajjakul, P., Wongwit, W., Punprasit, W., Eampokalap, B., Peacock, S., Ramasoota, P. Southeast Asian J. Trop. Med. Public Health (2005) [Pubmed]
  2. Gyrase mutations in laboratory-selected, fluoroquinolone-resistant mutants of Mycobacterium tuberculosis H37Ra. Kocagöz, T., Hackbarth, C.J., Unsal, I., Rosenberg, E.Y., Nikaido, H., Chambers, H.F. Antimicrob. Agents Chemother. (1996) [Pubmed]
  3. The species Mycobacterium africanum in the light of new molecular markers. Niemann, S., Kubica, T., Bange, F.C., Adjei, O., Browne, E.N., Chinbuah, M.A., Diel, R., Gyapong, J., Horstmann, R.D., Joloba, M.L., Meyer, C.G., Mugerwa, R.D., Okwera, A., Osei, I., Owusu-Darbo, E., Schwander, S.K., Rüsch-Gerdes, S. J. Clin. Microbiol. (2004) [Pubmed]
  4. Study of the gyrB gene polymorphism as a tool to differentiate among Mycobacterium tuberculosis complex subspecies further underlines the older evolutionary age of 'Mycobacterium canettii'. Goh, K.S., Fabre, M., Huard, R.C., Schmid, S., Sola, C., Rastogi, N. Mol. Cell. Probes (2006) [Pubmed]
  5. Characterization of pyrazinamide and ofloxacin resistance among drug resistant Mycobacterium tuberculosis isolates from Singapore. Lee, A.S., Tang, L.L., Lim, I.H., Wong, S.Y. Int. J. Infect. Dis. (2002) [Pubmed]
  6. DNA gyrase genes in Mycobacterium tuberculosis: a single operon driven by multiple promoters. Unniraman, S., Chatterji, M., Nagaraja, V. J. Bacteriol. (2002) [Pubmed]
  7. Molecular cloning of gyrA and gyrB genes of Mycobacterium tuberculosis: analysis of nucleotide sequence. Madhusudan, K., Ramesh, V., Nagaraja, V. Biochem. Mol. Biol. Int. (1994) [Pubmed]
 
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