Disease relevance of CCNF

• A novel cyclin gene (CCNF) in the region of the polycystic kidney disease gene (PKD1) [1].
• In this review the role of multiparameter analyses of cell cycle regulatory proteins in breast cancer will be outlined with special attention to pattern analyses as well as the definition of two contrasting pathways in tumorigenesis defined by either cyclin D1 or cyclin F overexpression [2].

High impact information on CCNF

• Cyclin F is the largest cyclin, with a molecular weight of 87 kDa, and migrates as a 100-110 kDa protein [3].
• Cyclin F protein accumulates in interphase and is destroyed at mitosis at a time distinct from cyclin B. Cyclin F shows regulated subcellular localization, being localized in the nucleus in most cells, with a significant percentage of cells displaying only perinuclear staining [3].
• Overexpression of cyclin F, or a mutant lacking the PEST region, in human cells resulted in a significant increase in the G2 population, implicating cyclin F in the regulation of cell cycle transitions [3].
• Rapid destruction of cyclin F does not require the N-terminal F-box motif but requires the COOH-terminal PEST sequences [4].
• Here we show that, similar to cyclin A, cyclin F is degraded when the spindle assembly checkpoint is activated and accumulates when the DNA damage checkpoint is activated [4].

Biological context of CCNF

• The ubiquitous expression and phylogentic conservation of cyclin F suggests that it is likely to coordinate essential cell cycle events distinct from those regulated by other cyclins [3].

Anatomical context of CCNF

• The G2 arrest in both cell lines was likely due to repression of PLK1 and/or CCNF [5].

References