High impact information on F-Box Motifs

• We cloned SON1 and found it to encode a novel protein containing an F-box motif, an element found within the specificity determinant in the E3 ubiquitin-ligase complex [1].
• Map-based cloning of ORE9 identified a 693-amino acid polypeptide containing an F-box motif and 18 leucine-rich repeats [2].
• Rapid destruction of cyclin F does not require the N-terminal F-box motif but requires the COOH-terminal PEST sequences [3].
• In addition, the chimerical proteins with swapped domain of the F-box motif or LRR between GmCOI1 and COI1 were shown to functionally complement the coil-1 mutation [4].
• However, the most interesting and surprising finding in this work was that the related RH proteins carry an F-box motif [5].

Associations of F-Box Motifs with chemical compounds

• In this study, we isolated an F-box protein gene from soybean, which shares significant homology with the Arabidopsis COI1 and similarly contains an F-box motif and leucine rich repeats (LRR), here designated GmCOI1 (Glycine max L. (Merr.) COI1) [4].

Gene context of F-Box Motifs

• F-box proteins bind Skp1 through the F-box motif, and substrates by means of carboxy-terminal protein interaction domains [6].
• The F-box motif of the At5g48990 gene product, a member of the family, was shown to be functionally active by its ability to mediate the in vitro interaction between At5g48990 and ASK1 proteins [7].
• Interaction with components of the SCF complex is mediated through the F-box motif of the F-box protein while it associates with phosphorylated substrates through its second protein-protein interaction motif such as Trp-Asp (WD) repeats or leucine-rich repeats (LRRs) [8].

References