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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review


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Disease relevance of Interphase


High impact information on Interphase

  • As the copy number of Rb9 increases, the association with the interphase nucleolus of the rDNA repeats centromeric to the Apc locus on Chromosome 18 is increasingly disrupted [6].
  • Inscuteable and Staufen interact and colocalize with prospero RNA on the apical cortex of interphase neuroblasts [7].
  • We observed a striking preferential but not exclusive association of Drosophila ORC2 with heterochromatin on interphase and mitotic chromosomes [8].
  • Unexpectedly, Ikaros localized to discrete heterochromatin-containing foci in interphase nuclei, which comprise clusters of centromeric DNA as defined by gamma-satellite sequences and the abundance of heterochromatin protein-1 (HP-1) [9].
  • Xcdc6 binds to chromatin at the beginning of interphase but disappears from chromatin upon initiation of replication [10].

Chemical compound and disease context of Interphase


Biological context of Interphase

  • We have aligned the molecular map of the Notch locus to the cytological features of the salivary gland polytene chromosomes of D. melanogaster in order to determine the interphase chromatin structure of this gene [16].
  • Mutations at the string (stg) locus cause cell-cycle arrest during this transition, in G2 of interphase 14, yet do not arrest other aspects of development [17].
  • The kinase activity of pp60c-src is specifically and transiently increased during mitosis and repressed during interphase [18].
  • Inhibition of phosphatases with okadaic acid in interphase cells leads to the phosphorylation of c-Abl mitotic sites, indicating that those sites are preferentially dephosphorylated during interphase [19].
  • Specifically, if p9 is removed from interphase egg extracts, these p9-depleted extracts are unable to carry out the proteolysis of cyclin B after entry into mitosis and thus remain arrested in M phase [20].

Anatomical context of Interphase


Associations of Interphase with chemical compounds

  • The high transcriptional activity characteristic of cycle 14 can be precociously induced by extending interphase with cycloheximide as early as, but not before, cycle 10 [26].
  • Small nuclear ribonucleoprotein particles (snRNPs) and non-snRNP splicing factors containing a serine/arginine-rich domain (SR proteins) concentrate in 'speckles' in the nucleus of interphase cells [27].
  • A definitive defect of interphase is abolishment of Cds1 (a checkpoint kinase) activation in the presence of hydroxyurea in both cnd2-1 mutant cells and in cells where other condensin subunits have been genetically disrupted [28].
  • Nocodazole itself has no effect on junctional coupling in interphase cells, regardless of the extent of intercellular flattening, whereas taxol, a microtubule-stabilizing agent, does reduce the extent of coupling in interphase cells [29].
  • Using bromodeoxyuridine incorporation and DNA quantitation in combination with fluorescence in situ hybridization, we detected gross chromosomal movements in diploid interphase nuclei of larvae [30].

Gene context of Interphase

  • Addition of the PRAD1 protein to interphase clam embryo lysates containing inactive p34cdc2 kinase and lacking endogenous cyclins allows it to be isolated using beads bearing p13suc1, a yeast protein that binds cdc2 and related kinases with high affinity and coprecipitates kinase-associated proteins [31].
  • Ablation of cyclin A messenger RNA in cytostatic factor/metaphase-arrested extracts of Xenopus eggs, followed by in vitro progression into interphase, resulted in the premature appearance of cyclin B-cdc2-associated H1 kinase activity and premature entry into mitosis [32].
  • Our results indicate that SRPK1 may have a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells, and the reorganization of nuclear speckles during mitosis [27].
  • Indirect immunofluoresence staining of interphase nuclei localized SETDB1 predominantly in euchromatic regions that overlap with HP1 staining in nonpericentromeric regions of chromatin [33].
  • Bmi1, M33, and Mph1 show an overlapping speckled distribution in interphase nuclei [34].

Analytical, diagnostic and therapeutic context of Interphase


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  7. Inscuteable and Staufen mediate asymmetric localization and segregation of prospero RNA during Drosophila neuroblast cell divisions. Li, P., Yang, X., Wasser, M., Cai, Y., Chia, W. Cell (1997) [Pubmed]
  8. Association of the origin recognition complex with heterochromatin and HP1 in higher eukaryotes. Pak, D.T., Pflumm, M., Chesnokov, I., Huang, D.W., Kellum, R., Marr, J., Romanowski, P., Botchan, M.R. Cell (1997) [Pubmed]
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  10. The Xenopus Cdc6 protein is essential for the initiation of a single round of DNA replication in cell-free extracts. Coleman, T.R., Carpenter, P.B., Dunphy, W.G. Cell (1996) [Pubmed]
  11. Staining with pyronin Y detects changes in conformation of RNA during mitosis and hyperthermia of CHO cells. Traganos, F., Crissman, H.A., Darzynkiewicz, Z. Exp. Cell Res. (1988) [Pubmed]
  12. Microtubule disruption induced by estradiol in estrogen receptor-positive and -negative human breast cancer cell lines. Aizu-Yokota, E., Ichinoseki, K., Sato, Y. Carcinogenesis (1994) [Pubmed]
  13. Identification of triploidy by DA/DAPI staining of trophoblastic interphase nuclei. Tommerup, N., Vejerslev, L.O. Placenta (1985) [Pubmed]
  14. Technical note: comparison of yields and repair kinetics of interphase chromosome breaks visualized by Sendai-virus or PEG-mediated cell fusion in irradiated CHO cells. Okayasu, R., Cheong, N., Iliakis, G. Int. J. Radiat. Biol. (1993) [Pubmed]
  15. Localisation of DNA topoisomerase IIalpha in mouse erythroleukemia cells. Ivanova, E.C., Donev, R.M., Djondjurov, L.P. Mol. Cells (1999) [Pubmed]
  16. Precise determination of the molecular limits of a polytene chromosome band: regulatory sequences for the Notch gene are in the interband. Rykowski, M.C., Parmelee, S.J., Agard, D.A., Sedat, J.W. Cell (1988) [Pubmed]
  17. Genetic control of cell division patterns in the Drosophila embryo. Edgar, B.A., O'Farrell, P.H. Cell (1989) [Pubmed]
  18. Cell transformation and activation of pp60c-src by overexpression of a protein tyrosine phosphatase. Zheng, X.M., Wang, Y., Pallen, C.J. Nature (1992) [Pubmed]
  19. Differential phosphorylation of c-Abl in cell cycle determined by cdc2 kinase and phosphatase activity. Kipreos, E.T., Wang, J.Y. Science (1990) [Pubmed]
  20. Xe-p9, a Xenopus Suc1/Cks protein, is essential for the Cdc2-dependent phosphorylation of the anaphase- promoting complex at mitosis. Patra, D., Dunphy, W.G. Genes Dev. (1998) [Pubmed]
  21. The nuclear envelope lamina is reversibly depolymerized during mitosis. Gerace, L., Blobel, G. Cell (1980) [Pubmed]
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  23. Small splenic B cells that bind to antigen-specific T helper (Th) cells and face the site of cytokine production in the Th cells selectively proliferate: immunofluorescence microscopic studies of Th-B antigen-presenting cell interactions. Kupfer, H., Monks, C.R., Kupfer, A. J. Exp. Med. (1994) [Pubmed]
  24. Centrosomes and the Scrambled protein coordinate microtubule-independent actin reorganization. Stevenson, V.A., Kramer, J., Kuhn, J., Theurkauf, W.E. Nat. Cell Biol. (2001) [Pubmed]
  25. Spectrin redistributes to the cytosol and is phosphorylated during mitosis in cultured cells. Fowler, V.M., Adam, E.J. J. Cell Biol. (1992) [Pubmed]
  26. Parameters controlling transcriptional activation during early Drosophila development. Edgar, B.A., Schubiger, G. Cell (1986) [Pubmed]
  27. A serine kinase regulates intracellular localization of splicing factors in the cell cycle. Gui, J.F., Lane, W.S., Fu, X.D. Nature (1994) [Pubmed]
  28. Cnd2 has dual roles in mitotic condensation and interphase. Aono, N., Sutani, T., Tomonaga, T., Mochida, S., Yanagida, M. Nature (2002) [Pubmed]
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  30. Large-scale chromosomal movements during interphase progression in Drosophila. Csink, A.K., Henikoff, S. J. Cell Biol. (1998) [Pubmed]
  31. A novel cyclin encoded by a bcl1-linked candidate oncogene. Motokura, T., Bloom, T., Kim, H.G., Jüppner, H., Ruderman, J.V., Kronenberg, H.M., Arnold, A. Nature (1991) [Pubmed]
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  33. SETDB1: a novel KAP-1-associated histone H3, lysine 9-specific methyltransferase that contributes to HP1-mediated silencing of euchromatic genes by KRAB zinc-finger proteins. Schultz, D.C., Ayyanathan, K., Negorev, D., Maul, G.G., Rauscher, F.J. Genes Dev. (2002) [Pubmed]
  34. Identification of Bmi1-interacting proteins as constituents of a multimeric mammalian polycomb complex. Alkema, M.J., Bronk, M., Verhoeven, E., Otte, A., van 't Veer, L.J., Berns, A., van Lohuizen, M. Genes Dev. (1997) [Pubmed]
  35. Proximity of chromosomal loci that participate in radiation-induced rearrangements in human cells. Nikiforova, M.N., Stringer, J.R., Blough, R., Medvedovic, M., Fagin, J.A., Nikiforov, Y.E. Science (2000) [Pubmed]
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  37. Human ECT2 is an exchange factor for Rho GTPases, phosphorylated in G2/M phases, and involved in cytokinesis. Tatsumoto, T., Xie, X., Blumenthal, R., Okamoto, I., Miki, T. J. Cell Biol. (1999) [Pubmed]
  38. Erythrocyte ankyrin: immunoreactive analogues are associated with mitotic structures in cultured cells and with microtubules in brain. Bennett, V., Davis, J. Proc. Natl. Acad. Sci. U.S.A. (1981) [Pubmed]
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