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Gene Review:

fdnG - formate dehydrogenase-N, alpha subunit,...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1468, JW1470

Berg, B.L. et al., Berg, B.L. et al., Ringquist, S. et al., Rabin, R.S. et al.

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Disease relevance of fdnG

fdnG, encoding the selenopeptide of Escherichia coli formate dehydrogenase-N, contains an in-frame opal (UGA) codon at amino acid position 196 that directs selenocysteine incorporation [1].

High impact information on fdnG

Boundary and toeprint experiments illustrate that the SELB-GTP-Sec-tRNA(Sec) ternary complex binds to the selenoprotein encoding mRNAs fdhF and fdnG, serving to increase the concentration of SELB and Sec-tRNA(Sec) on these mRNAs in vivo [2].
The sequence contains three open reading frames of sizes appropriate to encode the three subunits of formate dehydrogenase-N. fdnG contains an in-frame UGA codon that specifies selenocysteine incorporation, and the predicted amino acid sequence of FdnG shows similarity to two other bacterial formate dehydrogenase enzymes [3].
Our data indicate that both stability and specific nucleotide sequences of the stem-loop likely contribute to the appropriate mRNA context for selenocysteine incorporation into the fdnG gene product [1].
First, narL null mutants retain residual nitrate induction of fdnG operon expression; this residual induction is absent in narX narQ double-null strains [4].

References

Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. II. Evidence that a mRNA stem-loop structure is essential for decoding opal (UGA) as selenocysteine. Berg, B.L., Baron, C., Stewart, V. J. Biol. Chem. (1991) [Pubmed]
Recognition of the mRNA selenocysteine insertion sequence by the specialized translational elongation factor SELB. Ringquist, S., Schneider, D., Gibson, T., Baron, C., Böck, A., Gold, L. Genes Dev. (1994) [Pubmed]
Nitrate-inducible formate dehydrogenase in Escherichia coli K-12. I. Nucleotide sequence of the fdnGHI operon and evidence that opal (UGA) encodes selenocysteine. Berg, B.L., Li, J., Heider, J., Stewart, V. J. Biol. Chem. (1991) [Pubmed]
Dual response regulators (NarL and NarP) interact with dual sensors (NarX and NarQ) to control nitrate- and nitrite-regulated gene expression in Escherichia coli K-12. Rabin, R.S., Stewart, V. J. Bacteriol. (1993) [Pubmed]

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