High impact information on hipB

• Furthermore, expression of hipA7 in the absence of hipB is sufficient to establish the high persistent phenotype, indicating that hipB is not required [1].
• In contrast, expression of the mutant hipA7 gene in excess of hipB did not markedly inhibit protein synthesis as did wild-type hipA and yet still conferred persistence to ca. 10% of cells [2].
• Moreover, hipA-containing plasmids cannot be replicated in strains defective in or lacking hipB [3].

Biological context of hipB

• The stop codon of a 264-bp open reading frame, hipB, and the start codon of a 1,320-bp open reading frame, hipA, share an adenine residue [4].
• Regulation of the hip operon, which consists of a regulatory region and two genes, hipB and hipA, was examined with strains containing a hip-lac transcriptional fusion placed in single copy at the lambda att site [3].

Analytical, diagnostic and therapeutic context of hipB

• The genes hipA and hipB were amplified by PCR from persisters and sequenced [5].

References