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Gene Review:

chbA - N,N'-diacetylchitobiose-specific enzyme...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1734, JW1725, celC

Xue, G.P. et al., Reverbel-Leroy, C. et al., Li, X.L. et al., Parker, L.L. et al., Fukumori, F. et al.

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Disease relevance of chbA

Furthermore, the celC encoded PEP-dependent phosphotransferase system enzyme IIIcel is strikingly homologous to an enzyme IIIlac of the Gram-positive organism Staphylococcus aureus [1].
The gene lies between cipC (the gene encoding the cellulosome scaffolding protein) and celC (coding for the endoglucanase C) in the large cel cluster of this mesophilic cellulolytic Clostridium species [2].

High impact information on chbA

Nucleotide sequencing revealed that the celA cDNA (1,558 bp) and celC cDNA (1,628 bp) had open reading frames encoding polypeptides of 459 (CelA) and 449 (CelC) amino acids, respectively [3].
The third cellulase gene (celC) of Bacillus sp. strain N-4 was cloned in plasmid pBR322 and was located within a 5.5-kb HindIII fragment [4].
Studies on the substrate specificity showed that the enzyme encoded by celA had high activity towards amorphous and microcrystalline cellulose, while the celB and celC enzymes had relatively high activity on carboxymethylcellulose, with little activity on microcrystalline cellulose [5].

References

Characterization and nucleotide sequence of the cryptic cel operon of Escherichia coli K12. Parker, L.L., Hall, B.G. Genetics (1990) [Pubmed]
Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli. Reverbel-Leroy, C., Belaich, A., Bernadac, A., Gaudin, C., Belaich, J.P., Tardif, C. Microbiology (Reading, Engl.) (1996) [Pubmed]
Two cellulases, CelA and CelC, from the polycentric anaerobic fungus Orpinomyces strain PC-2 contain N-terminal docking domains for a cellulase-hemicellulase complex. Li, X.L., Chen, H., Ljungdahl, L.G. Appl. Environ. Microbiol. (1997) [Pubmed]
The third cellulase of alkalophilic Bacillus sp. strain N-4: evolutionary relationships within the cel gene family. Fukumori, F., Kudo, T., Sashihara, N., Nagata, Y., Ito, K., Horikoshi, K. Gene (1989) [Pubmed]
Cloning and expression of multiple cellulase cDNAs from the anaerobic rumen fungus Neocallimastix patriciarum in Escherichia coli. Xue, G.P., Orpin, C.G., Gobius, K.S., Aylward, J.H., Simpson, G.D. J. Gen. Microbiol. (1992) [Pubmed]

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