Disease relevance of fliP

• fliP influences Citrobacter koseri macrophage uptake, cytokine expression and brain abscess formation in the neonatal rat [1].
• We report the identification of a previously unknown Listeria monocytogenes gene, flaR, which modulates DNA topology [2].
• When expressed in Escherichia coli, flaR encodes a protein of 18 kDa (FlaR) whose transcription is osmoregulated [2].

High impact information on fliP

• The transposon had inserted in flaR, an open reading frame of 531 bp, followed by a second open reading frame of 1252 bp in reverse orientation [2].
• On the basis of DNA sequence analysis and in vivo protein expression, it appears that the fliP gene product is synthesized as a precursor protein with an N-terminal signal peptide of 21 amino acids [3].
• In minicell preparations, the flaAII.3 and flaR products were found principally in the cytoplasmic fraction; the rest were found principally in the membrane fraction [4].
• The flaR promoter region contains putative LysR binding motifs and two putative Fur boxes [5].

Analytical, diagnostic and therapeutic context of fliP

• The entire gene, cloned by inverse PCR, was named flaR [5].

References