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Gene Review:

wzxB - putative polisoprenol-linked O-antigen...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2031, JW2022, rfbX, wzx

Macpherson, D.F. et al., Klena, J.D. et al., Burrows, L.L. et al.

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Disease relevance of rfbX

Genetic analysis of the rfbX gene of Shigella flexneri [1].
The rfbX gene encodes a hydrophobic, 46-kDa protein, with 12 potential transmembrane-spanning domains, that shows structural homology with gene products encoded in many rfb regions, and with Orf0416 of the rff region of Escherichia coli K-12 which has also been identified as a member of this class of proteins [1].
Effect of wzx (rfbX) mutations on A-band and B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5 [2].

High impact information on rfbX

Eight genes were identified in the rfb fragment: the rfbB-CAD cluster which encodes dTDP-rhamnose synthesis, rfbX which encodes a hydrophobic protein involved in assembly of the O antigen, rfc which encodes the O antigen polymerase, and two sugar transferase genes [3].
Analysis of the nucleotide sequence of the rfbX gene of Shigella flexneri revealed that it contained a high proportion of rare codons, as previously observed in the analysis of the O-antigen polymerase-encoding gene rfc [Morona et al., J. Bacteriol. 176 (1994) 733-747] [1].
Analysis of plasmids containing various deletions within the rfb region suggest that the 5' end of rfbX plays an indirect regulatory role in expression of the dTDP-rhamnose biosynthetic enzymes, encoded by rfbBCAD [1].

References

Genetic analysis of the rfbX gene of Shigella flexneri. Macpherson, D.F., Manning, P.A., Morona, R. Gene (1995) [Pubmed]
Effect of wzx (rfbX) mutations on A-band and B-band lipopolysaccharide biosynthesis in Pseudomonas aeruginosa O5. Burrows, L.L., Lam, J.S. J. Bacteriol. (1999) [Pubmed]
Function of the rfb gene cluster and the rfe gene in the synthesis of O antigen by Shigella dysenteriae 1. Klena, J.D., Schnaitman, C.A. Mol. Microbiol. (1993) [Pubmed]

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