The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

mscL  -  mechanosensitive channel protein, high...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3277, JW3252, stkA, yhdC
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of mscL

 

High impact information on mscL

  • Insertional disruption of mscL removes the channel activity, whereas re-expression of mscL borne on an expression plasmid restores it [1].
  • The mscL nucleotide sequence predicts a unique protein of only 136 amino acids, with a highly hydrophobic core and very different from porins or other known proteins [1].
  • One gene, mscL, encoding a large conductance channel has previously been described, but null mutants were without well-defined phenotypes [4].
  • Using chromatographies to enrich the target and patch clamp to assay the channel activity in liposome-reconstituted fractions, we identified the MscL protein and cloned the mscL gene [5].
  • Reduced levels of MS proteins and reduced expression of mscL- and mscS-LacZ fusions in an rpoS mutant strain suggested that the RNA polymerase holoenzyme containing sigmaS is responsible, at least in part, for regulating production of MS channel proteins [6].

References

  1. A large-conductance mechanosensitive channel in E. coli encoded by mscL alone. Sukharev, S.I., Blount, P., Martinac, B., Blattner, F.R., Kung, C. Nature (1994) [Pubmed]
  2. Identification of the gene encoding a mechanosensitive channel MscL homologue in Clostridium perfringens. Matsushita, O., Jung, C.M., Okabe, A. Gene (1995) [Pubmed]
  3. Mechanosensitive channel functions to alleviate the cell lysis of marine bacterium, Vibrio alginolyticus, by osmotic downshock. Nakamaru, Y., Takahashi, Y., Unemoto, T., Nakamura, T. FEBS Lett. (1999) [Pubmed]
  4. Protection of Escherichia coli cells against extreme turgor by activation of MscS and MscL mechanosensitive channels: identification of genes required for MscS activity. Levina, N., Tötemeyer, S., Stokes, N.R., Louis, P., Jones, M.A., Booth, I.R. EMBO J. (1999) [Pubmed]
  5. Mechanosensitive channels of Escherichia coli: the MscL gene, protein, and activities. Sukharev, S.I., Blount, P., Martinac, B., Kung, C. Annu. Rev. Physiol. (1997) [Pubmed]
  6. A role for mechanosensitive channels in survival of stationary phase: regulation of channel expression by RpoS. Stokes, N.R., Murray, H.D., Subramaniam, C., Gourse, R.L., Louis, P., Bartlett, W., Miller, S., Booth, I.R. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
 
WikiGenes - Universities