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Gene Review:

trpT - tRNA

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3755, JWR0087, su7, su8, su9, ...

Schultz, D.W. et al., Raftery, L.A. et al., Gray, J.E. et al.

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High impact information on trpT

Following saturating mutagenesis of the anticodon arm of su7 G36, screening for UAG suppression using a lacZ reporter yielded tRNAs with up to 40-fold increased first position G-U wobble, judged from KUAG [1].
Measurement of amber suppression by mutated su7 G36 tRNAs and correction for tRNA levels and aminoacylation allowed calculation of KUAG, a linearized index of in vivo ribosomal function [1].
Transfer RNA su7 G36 is a derivative of tRNA(Trp) with a 3'GUC anticodon complementary to the glutamine codon CAG [1].
Data on the DNA sequences of these trpT alleles and on amino acid specificity of the suppressors are presented [2].
The construction of the Su7 -UAG plasmid and the wild-type trpT plasmid have been previously described ( Yarus , et al., Proc. Natl. Acad. Sci. U.S.A. 77:5092-5097, 1980) [2].

Biological context of trpT

The gene coding for the 15,000-dalton protein was located within a region about 1,000 base pairs long between ilv and trpT [3].

References

tRNA structure and ribosomal function. I. tRNA nucleotide 27-43 mutations enhance first position wobble. Schultz, D.W., Yarus, M. J. Mol. Biol. (1994) [Pubmed]
Defined set of cloned termination suppressors: in vivo activity of isogenetic UAG, UAA, and UGA suppressor tRNAs. Raftery, L.A., Egan, J.B., Cline, S.W., Yarus, M. J. Bacteriol. (1984) [Pubmed]
Identification of a protein of 15,000 daltons related to isoleucine-valine biosynthesis in Escherichia coli K-12. Gray, J.E., Wallen, J.W., Calhoun, D.H. J. Bacteriol. (1982) [Pubmed]

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