Disease relevance of RNA-1

• We have cloned the region of tomato ringspot nepovirus (TomRSV) RNA-1 coding for the putative TomRSV 3C-related protease (amino acids 1213 to 1508) in a transcription vector and in a transient expression vector [1].

High impact information on RNA-1

• In contrast, only one protein domain is present in the equivalent region of the P2 polyprotein of other characterized nepoviruses [2].
• Tomato ringspot nepovirus (TomRSV) RNA-1 encodes a putative NTP-binding protein (NTB), a putative viral genome-linked protein (VPg), a putative RNA-dependent RNA polymerase (Pol) and a serine-like protease (Pro), which have been suggested to be involved in viral RNA replication [3].
• Nucleotide sequence of tomato ringspot virus RNA1 [4].
• TomRSV RNA1 is 8214 nucleotides in length, excluding the 3' poly(A) tail, and contains a single long open reading frame (ORF) of 6591 nucleotides beginning at the first AUG codon at nucleotide position 78 [4].
• Amino acid sequence comparisons between portions of the TomRSV RNA1-encoded polyprotein and proteins encoded by several members of the picornavirus superfamily have provided information concerning the genomic organization and putative functions of TomRSV-encoded proteins [4].

Biological context of RNA-1

• Sequence comparison of the tomato ringspot nepovirus (TomRSV) genome with related viruses suggested that the region of the RNA-2-encoded polyprotein immediately upstream of the coat protein may be involved in the cell-to-cell movement of the virus (Rott et al., 1991, J. Gen. Virol. 72, 1505-1514) [5].
• The biological function of the long 3' non-coding region and how the high level of sequence homology is maintained between RNA1 and RNA2, is unknown [6].

Analytical, diagnostic and therapeutic context of RNA-1

• Sequence analysis of the 3' termini of RNA1 and RNA2 of blueberry leaf mottle virus [6].

References