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Gene Review:

sbm - methylmalonyl-CoA mutase

Escherichia coli O157:H7 str. EDL933

Bobik, T.A. et al., Ratnatilleke, A. et al., Kennedy, J. et al., Abo, T. et al.

Bobik, Rasche,

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Disease relevance of sbm

To obtain the methylmalonyl-CoA mutase which was required as a reagent for the assay, an Escherichia coli strain was constructed that expressed high levels of this enzyme as a fusion protein with an 8x histidine tag [1].

High impact information on sbm

Isobutyryl-CoA mutase (ICM) catalyzes the reversible, coenzyme B(12)-dependent rearrangement of isobutyryl-CoA to n-butyryl-CoA, which is similar to, but distinct from, that catalyzed by methylmalonyl-CoA mutase [2].
The DEBS polyketide synthase (PKS) used butyryl-CoA and methylmalonyl-CoA supplied in vivo by the AtoAD and methylmalonyl-CoA mutase pathways, respectively, to produce 15-methyl-6-dEB [3].
This region (designated sbm) was located within the nonconserved region in oriT among conjugative plasmids related to R100 [4].

References

HPLC assay for methylmalonyl-CoA epimerase. Bobik, T.A., Rasche, M.E. Analytical and bioanalytical chemistry. (2003) [Pubmed]
Cloning and sequencing of the coenzyme B(12)-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis, reconstitution of mutase activity, and characterization of the recombinant enzyme produced in Escherichia coli. Ratnatilleke, A., Vrijbloed, J.W., Robinson, J.A. J. Biol. Chem. (1999) [Pubmed]
6-Deoxyerythronolide B analogue production in Escherichia coli through metabolic pathway engineering. Kennedy, J., Murli, S., Kealey, J.T. Biochemistry (2003) [Pubmed]
Specific DNA binding of the TraM protein to the oriT region of plasmid R100. Abo, T., Inamoto, S., Ohtsubo, E. J. Bacteriol. (1991) [Pubmed]

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