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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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A PCR assay for discriminating Neisseria gonorrhoeaebeta-lactamase-producing plasmids.

Oligonucleotide primers were developed for use in polymerase chain reaction (PCR) assays to differentiate three related, epidemic beta-lactamase-producing plasmids of Neisseria gonorrhoeae-the Asia-(7426 bp), Africa-(5599 bp) and Toronto-(5154 bp) type plasmids. One-hundred and two N. gonorrhoeae isolates with different plasmid profiles were tested-16 isolates carried the Asia plasmid, 41 isolates contained the Africa plasmid, 16 isolates contained the Toronto plasmid and 29 isolates contained no beta-lactamase-producing plasmids. Most (101/102) isolates also carried the gonococcal cryptic plasmid, while 27/102 and 44/102 isolates carried either the transfer plasmid or the tet M-containing plasmids, respectively. The assay was 100% sensitive and specific for identifying the correct plasmid type. This assay is useful for rapidly detecting the presence of gonococcal beta-lactamase-producing plasmids in clinical samples and discriminating them for epidemiological typing.[1]

References

  1. A PCR assay for discriminating Neisseria gonorrhoeaebeta-lactamase-producing plasmids. Dillon, J.R., Li, H., Yeung, K., Aman, T.A. Mol. Cell. Probes (1999) [Pubmed]
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