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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
 
 
 
 

Inducible isopentenyl transferase as a high-efficiency marker for plant transformation.

Overexpression of the isopentenyltransferase gene ( ipt) from the Ti-plasmid of Agrobacterium tumefaciens increases cytokinin levels, leading to generation of shoots from transformed plant cells. When combined with a dexamethasone-inducible system for controlling expression, ipt expression can be used to select for transgenic regenerants without using an antibiotic-resistance marker. The combined system allows efficient cointroduction of multiple genes (in addition to ipt) and produces transgenic plants without morphological or developmental defects.[1]

References

  1. Inducible isopentenyl transferase as a high-efficiency marker for plant transformation. Kunkel, T., Niu, Q.W., Chan, Y.S., Chua, N.H. Nat. Biotechnol. (1999) [Pubmed]
 
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