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Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000.

The complete 42180-bp nucleotide sequence of the mobilization plasmid pNZ4000, coding for exopolysaccharide (EPS) production in Lactococcus lactis, was determined. This plasmid contains a region involved in EPS biosynthesis, four functional replicons, a region containing mobilization genes, and three origin of transfer (oriT) sequences. Sequences identical to these oriT sequences were also found on two other lactococcal plasmids and a plasmid from Lactobacillus helveticus. Several complete and partial IS elements were identified on pNZ4000, including iso-ISS1, iso-IS946, and iso-IS982 sequences. Furthermore, pNZ4000 contains a gene cluster that may encode a cobalt transport system and a gene that encodes a CorA homologue which may function as a magnesium transporter.[1]

References

  1. Nucleotide sequence analysis of the lactococcal EPS plasmid pNZ4000. van Kranenburg, R., Kleerebezem, M., de Vos, W.M. Plasmid (2000) [Pubmed]
 
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