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Nkengasong, J.N. et al.

Nkengasong, Luo, Abouya, Pieniazek, Maurice, Sassan-Morokro, Ellenberger, Hu, Pau, Dobbs, Respess, Coulibaly, Coulibaly, Wiktor, Greenberg, Rayfield,

Distribution of HIV-1 subtypes among HIV-seropositive patients in the interior of CÃ´te d'Ivoire.

Limited data exist on the distribution of HIV-1 subtypes in CÃ´te d'Ivoire. The aim of this study is to describe the distribution of genetic subtypes of HIV-1 strains in six regions of CÃ´te d'Ivoire. In 1997, we consecutively collected blood from 172 HIV-1-infected patients from six regional tuberculosis treatment centers. Peripheral blood mononuclear cells (PBMCs) from these people were analyzed by a restriction fragment-length polymorphism (RFLP) assay that involves a sequential endonuclease digestion of a 297-base pair polymerase chain reaction (PCR) fragment; plasma samples were tested by a V3-loop peptide enzyme immunoassay (PEIA). DNA sequencing of the protease or env genes was performed on all samples discordant in the two assays as well as a random sample of the concordant subtyped samples. Of 172 specimens, 3 were PCR-negative, and 169 were putatively classified as subtype A by RFLP. The 3 PCR-negative samples were unequivocally subtyped A by PEIA. Of the 169 RFLP subtype A samples, 159 (94%) were subtyped A by PEIA. Of the 10 discordant samples, PEIA testing classified 3 as subtype C, 2 as D, and 5 as F. Sequencing of the env gene classified these samples as 1 subtype A, 4 Ds, and 5 Gs. Thus, 163 (95%) of the specimens were subtype A, 3 subtype D, 4 subtype G, 1 A/D, and 1 A/G (IbNG) circulating recombinant forms (CRF). In conclusion, most HIV-1-infected tuberculosis patients throughout the interior of CÃ´te d'Ivoire are infected with HIV-1 subtype A, which are very likely the A/G (IbNG) CRF. The uniform distribution of this subtype makes CÃ´te d'Ivoire a potential site for vaccine trials.[1]

References

Distribution of HIV-1 subtypes among HIV-seropositive patients in the interior of Côte d'Ivoire. Nkengasong, J.N., Luo, C.C., Abouya, L., Pieniazek, D., Maurice, C., Sassan-Morokro, M., Ellenberger, D., Hu, D.J., Pau, C.P., Dobbs, T., Respess, R., Coulibaly, D., Coulibaly, I.M., Wiktor, S.Z., Greenberg, A.E., Rayfield, M. J. Acquir. Immune Defic. Syndr. (2000) [Pubmed]

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