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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 

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New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris.

We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris. The predicted products of the genes share significant sequence similarity to their Saccharomyces cerevisiae counterparts, namely argininosuccinate lyase, PR-aminoimidazolesuccinocarboxamide synthase, and orotidine-5'-phosphate decarboxylase, respectively. Along with the previously described HIS4 gene, each gene was incorporated as the yeast selectable marker into a set of shuttle vectors designed to express foreign genes in P. pastoris. In addition, we have constructed a series of host strains containing all possible combinations of ade1, arg4, his4, and ura3 auxotrophies to be used with these new vectors.[1]

References

  1. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Lin Cereghino, G.P., Lin Cereghino, J., Sunga, A.J., Johnson, M.A., Lim, M., Gleeson, M.A., Cregg, J.M. Gene (2001) [Pubmed]
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