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Disease relevance of Pichia


Psychiatry related information on Pichia


High impact information on Pichia

  • However, unique features of each pathway dictate the requirement for additional components that differentiate these pathways from one another, including at the step of specific cargo selection.We have identified Cvt9 and its Pichia pastoris counterpart Gsa9 [7].
  • Prion properties of the Sup35 protein of yeast Pichia methanolica [8].
  • We tested the interaction between Pex19p and all known Pichia pastoris Pex proteins by the yeast two-hybrid assay [9].
  • Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane [10].
  • We isolated a Pichia pastoris mutant that was unable to grow on the peroxisome-requiring media, methanol and oleate [10].

Chemical compound and disease context of Pichia


Biological context of Pichia

  • The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1 [16].
  • Expression of soluble ligand- and antibody-binding extracellular domain of human muscle acetylcholine receptor alpha subunit in yeast Pichia pastoris. Role of glycosylation in alpha-bungarotoxin binding [17].
  • Expression of the hexose oxidase cDNA in Pichia pastoris as an active enzyme confirmed the identity of the DNA sequence [18].
  • Furthermore, expression of fut-1 cDNA in Pichia and insect cells in conjunction with antibody staining, high pressure liquid chromatography, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analyses showed that FUT-1 is a core alpha1,3-fucosyltransferase with an unusual substrate specificity [19].
  • Previously, using the amine oxidase from the yeast Hansenula polymorpha (HPAO), mutations of a strictly conserved and structurally pivotal active site tyrosine (Y305) were studied and their effects on the catalytic cycle demonstrated [Hevel, J. M., Mills, S. A., and Klinman, J. P. (1999) Biochemistry 38, 3683-3693] [20].

Anatomical context of Pichia


Associations of Pichia with chemical compounds


Gene context of Pichia

  • Active NR protein was produced in a Pichia pastoris expression system [31].
  • We have cloned and characterized the Hansenula polymorpha PEX19 gene [32].
  • This B7-2(225)-scFv(FRP5) molecule expressed in the yeast Pichia pastoris and purified from culture supernatants is functionally active and binds to B7 counter-receptors and to ErbB2 [33].
  • To improve our understanding of the role that Pex17p plays in PMP import, we examined the behavior of PMPs in a Pichia pastoris pex17 mutant [34].
  • The use of artificial prion [PSI(+)PS] based on a hybrid Sup35PS with prion domain from the yeast Pichia methanolica allowed us to uncover three more chaperones, Ssb1, Ssa1, and Ydj1, whose overexpression can cure prion determinants [35].

Analytical, diagnostic and therapeutic context of Pichia


  1. Purification and characterization of the yeast-expressed erythropoietin mutant Epo (R103A), a specific inhibitor of human primary hematopoietic cell erythropoiesis. Burns, S., Arcasoy, M.O., Li, L., Kurian, E., Selander, K., Emanuel, P.D., Harris, K.W. Blood (2002) [Pubmed]
  2. Cloning, expression, purification, and characterization of the human broad specificity lysosomal acid alpha-mannosidase. Liao, Y.F., Lal, A., Moremen, K.W. J. Biol. Chem. (1996) [Pubmed]
  3. GMC oxidoreductases. A newly defined family of homologous proteins with diverse catalytic activities. Cavener, D.R. J. Mol. Biol. (1992) [Pubmed]
  4. Expression of functional influenza virus RNA polymerase in the methylotrophic yeast Pichia pastoris. Hwang, J.S., Yamada, K., Honda, A., Nakade, K., Ishihama, A. J. Virol. (2000) [Pubmed]
  5. Interaction between echovirus 7 and its receptor, decay-accelerating factor (CD55): evidence for a secondary cellular factor in A-particle formation. Powell, R.M., Ward, T., Evans, D.J., Almond, J.W. J. Virol. (1997) [Pubmed]
  6. Processing of the Alzheimer's disease amyloid precursor protein in Pichia pastoris: immunodetection of alpha-, beta-, and gamma-secretase products. Le Brocque, D., Henry, A., Cappai, R., Li, Q.X., Tanner, J.E., Galatis, D., Gray, C., Holmes, S., Underwood, J.R., Beyreuther, K., Masters, C.L., Evin, G. Biochemistry (1998) [Pubmed]
  7. Cvt9/Gsa9 functions in sequestering selective cytosolic cargo destined for the vacuole. Kim, J., Kamada, Y., Stromhaug, P.E., Guan, J., Hefner-Gravink, A., Baba, M., Scott, S.V., Ohsumi, Y., Dunn, W.A., Klionsky, D.J. J. Cell Biol. (2001) [Pubmed]
  8. Prion properties of the Sup35 protein of yeast Pichia methanolica. Kushnirov, V.V., Kochneva-Pervukhova, N.V., Chechenova, M.B., Frolova, N.S., Ter-Avanesyan, M.D. EMBO J. (2000) [Pubmed]
  9. The peroxin Pex19p interacts with multiple, integral membrane proteins at the peroxisomal membrane. Snyder, W.B., Koller, A., Choy, A.J., Subramani, S. J. Cell Biol. (2000) [Pubmed]
  10. Pex22p of Pichia pastoris, essential for peroxisomal matrix protein import, anchors the ubiquitin-conjugating enzyme, Pex4p, on the peroxisomal membrane. Koller, A., Snyder, W.B., Faber, K.N., Wenzel, T.J., Rangell, L., Keller, G.A., Subramani, S. J. Cell Biol. (1999) [Pubmed]
  11. Comparison of natural and recombinant isoforms of grass pollen allergens. Petersen, A., Grobe, K., Lindner, B., Schlaak, M., Becker, W.M. Electrophoresis (1997) [Pubmed]
  12. Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Liiv, L., Pärn, P., Alamäe, T. Gene (2001) [Pubmed]
  13. Sequencing and functional analysis of the Hansenula polymorpha genomic fragment containing the YPT1 and PMI40 genes. Kim, M.W., Agaphonov, M.O., Kim, J.Y., Rhee, S.K., Kang, H.A. Yeast (2002) [Pubmed]
  14. Antibody binding of deletion mutants of Asp f 2, the major Aspergillus fumigatus allergen. Tang, B., Banerjee, B., Greenberger, P.A., Fink, J.N., Kelly, K.J., Kurup, V.P. Biochem. Biophys. Res. Commun. (2000) [Pubmed]
  15. Recombinant expression of alliin lyase from garlic (Allium sativum) in bacteria and yeasts. Weik, R., Francky, A., Striedner, G., Raspor, P., Bayer, K., Mattanovich, D. Planta Med. (1998) [Pubmed]
  16. The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1. Waterham, H.R., de Vries, Y., Russel, K.A., Xie, W., Veenhuis, M., Cregg, J.M. Mol. Cell. Biol. (1996) [Pubmed]
  17. Expression of soluble ligand- and antibody-binding extracellular domain of human muscle acetylcholine receptor alpha subunit in yeast Pichia pastoris. Role of glycosylation in alpha-bungarotoxin binding. Psaridi-Linardaki, L., Mamalaki, A., Remoundos, M., Tzartos, S.J. J. Biol. Chem. (2002) [Pubmed]
  18. Hexose oxidase from the red alga Chondrus crispus. Purification, molecular cloning, and expression in Pichia pastoris. Hansen, O.C., Stougaard, P. J. Biol. Chem. (1997) [Pubmed]
  19. Molecular basis of anti-horseradish peroxidase staining in Caenorhabditis elegans. Paschinger, K., Rendic, D., Lochnit, G., Jantsch, V., Wilson, I.B. J. Biol. Chem. (2004) [Pubmed]
  20. Role of a strictly conserved active site tyrosine in cofactor genesis in the copper amine oxidase from Hansenula polymorpha. DuBois, J.L., Klinman, J.P. Biochemistry (2006) [Pubmed]
  21. The peroxisome biogenesis disorder group 4 gene, PXAAA1, encodes a cytoplasmic ATPase required for stability of the PTS1 receptor. Yahraus, T., Braverman, N., Dodt, G., Kalish, J.E., Morrell, J.C., Moser, H.W., Valle, D., Gould, S.J. EMBO J. (1996) [Pubmed]
  22. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule. Kishimoto, T.K., Jutila, M.A., Butcher, E.C. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  23. The role of mannosylated enzyme and the mannose receptor in enzyme replacement therapy. Du, H., Levine, M., Ganesa, C., Witte, D.P., Cole, E.S., Grabowski, G.A. Am. J. Hum. Genet. (2005) [Pubmed]
  24. Function and evolutionary conservation of distinct epitopes on the leukocyte adhesion molecule-1 (TQ-1, Leu-8) that regulate leukocyte migration. Spertini, O., Kansas, G.S., Reimann, K.A., Mackay, C.R., Tedder, T.F. J. Immunol. (1991) [Pubmed]
  25. Studies on the mechanism of 3-ketosphinganine synthetase. Krisnangkura, K., Sweeley, C.C. J. Biol. Chem. (1976) [Pubmed]
  26. Phosphomannosyl receptors may participate in the adhesive interaction between lymphocytes and high endothelial venules. Stoolman, L.M., Tenforde, T.S., Rosen, S.D. J. Cell Biol. (1984) [Pubmed]
  27. Assembly of human prolyl 4-hydroxylase and type III collagen in the yeast pichia pastoris: formation of a stable enzyme tetramer requires coexpression with collagen and assembly of a stable collagen requires coexpression with prolyl 4-hydroxylase. Vuorela, A., Myllyharju, J., Nissi, R., Pihlajaniemi, T., Kivirikko, K.I. EMBO J. (1997) [Pubmed]
  28. Structural basis of eukaryotic nitrate reduction: crystal structures of the nitrate reductase active site. Fischer, K., Barbier, G.G., Hecht, H.J., Mendel, R.R., Campbell, W.H., Schwarz, G. Plant Cell (2005) [Pubmed]
  29. Presence of a flavin semiquinone in methanol oxidase. Mincey, T., Tayrien, G., Mildvan, A.S., Abeles, R.H. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  30. The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene. Wimmer, B., Lottspeich, F., van der Klei, I., Veenhuis, M., Gietl, C. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  31. Identification in vitro of a post-translational regulatory site in the hinge 1 region of Arabidopsis nitrate reductase. Su, W., Huber, S.C., Crawford, N.M. Plant Cell (1996) [Pubmed]
  32. Hansenula polymorpha Pex19p is essential for the formation of functional peroxisomal membranes. Otzen, M., Perband, U., Wang, D., Baerends, R.J., Kunau, W.H., Veenhuis, M., Van der Klei, I.J. J. Biol. Chem. (2004) [Pubmed]
  33. Costimulation of T cell proliferation by a chimeric B7-2 antibody fusion protein specifically targeted to cells expressing the erbB2 proto-oncogene. Gerstmayer, B., Altenschmidt, U., Hoffmann, M., Wels, W. J. Immunol. (1997) [Pubmed]
  34. Peroxisomal membrane protein import does not require Pex17p. Harper, C.C., South, S.T., McCaffery, J.M., Gould, S.J. J. Biol. Chem. (2002) [Pubmed]
  35. Increased expression of Hsp40 chaperones, transcriptional factors, and ribosomal protein Rpp0 can cure yeast prions. Kryndushkin, D.S., Smirnov, V.N., Ter-Avanesyan, M.D., Kushnirov, V.V. J. Biol. Chem. (2002) [Pubmed]
  36. Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha. Ledeboer, A.M., Edens, L., Maat, J., Visser, C., Bos, J.W., Verrips, C.T., Janowicz, Z., Eckart, M., Roggenkamp, R., Hollenberg, C.P. Nucleic Acids Res. (1985) [Pubmed]
  37. Conserved surface-exposed K/R-X-K/R motifs and net positive charge on poxvirus complement control proteins serve as putative heparin binding sites and contribute to inhibition of molecular interactions with human endothelial cells: a novel mechanism for evasion of host defense. Smith, S.A., Mullin, N.P., Parkinson, J., Shchelkunov, S.N., Totmenin, A.V., Loparev, V.N., Srisatjaluk, R., Reynolds, D.N., Keeling, K.L., Justus, D.E., Barlow, P.N., Kotwal, G.J. J. Virol. (2000) [Pubmed]
  38. Cloning, expression, and clinical significance of the major allergen from ash pollen, Fra e 1. Barderas, R., Purohit, A., Papanikolaou, I., Rodríguez, R., Pauli, G., Villalba, M. J. Allergy Clin. Immunol. (2005) [Pubmed]
  39. Expression of group IA phospholipase A2 in Pichia pastoris: identification of a phosphatidylcholine activator site using site-directed mutagenesis. Lefkowitz, L.J., Deems, R.A., Dennis, E.A. Biochemistry (1999) [Pubmed]
  40. Purification and characterization of a neutral endopeptidase-like enzyme from human urine. Di Marco, G.S., Quinto, B.M., Juliano, M., Carmona, A.K., Stella, R.C., Plavnik, F.L., Casarini, D.E. J. Hypertens. (1998) [Pubmed]
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