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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
MeSH Review

Genetic Vectors

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Disease relevance of Genetic Vectors


High impact information on Genetic Vectors

  • When these were introduced into yeast on autonomously replicating shuttle vectors, a sequence element between 35 and 70 nucleotides upstream from the 3' splice site was required for splicing the actin gene transcripts [6].
  • A shuttle vector containing a regulated muscle-specific promoter has been developed for the stable expression of recombinant myosin in C2C12 cells [7].
  • A TAR cloning vector was constructed that, when linearized, contained a small amount (381 bp) of 3' hypoxanthine phosphoribosyltransferase (HPRT) sequence at one end and an 189-bp Alu repeat at the other end [8].
  • A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors [9].
  • The synthetic opsin gene was placed under the control of the inducible promoter GAL1 in the multicopy yeast/ Escherichia coli shuttle vector YEpRF1 [10].

Chemical compound and disease context of Genetic Vectors

  • A retroviral shuttle vector was constructed by introducing the Escherichia coli xanthine (guanine) phosphoribosyltransferase gene (gpt) into the pZip-NeoSV(X)1 vector [Cepko, C. L., Roberts, B. E. & Mulligan, R. C. (1984) Cell 37, 1053-1062] [11].
  • The homologous transfer of the bacterial ampicillin resistance gene from the inserted sequences to replace a mutant ampicillin sensitivity gene on the shuttle vector was identified by the recovery of ampicillin-resistant plasmids after Hirt extraction and transformation into Escherichia coli host cells [12].
  • We have devised a rapid four-step procedure for the generation of directed and unmarked chromosomal deletions in bacteria, based on the use of a novel cloning vector containing the Bacillus subtilis sacB gene that encodes levansucrase and confers sucrose sensitivity, which can be used for counter-selection [13].
  • The gene encoding a puromycin N-acetyl transferase from Streptomyces alboniger has been cloned next to the SV40 early promoter in a mammalian cells-Escherichia coli shuttle vector [14].
  • No significant high-salt DNA polymerase activity was detected in extracts from expresser cells grown in the presence of raffinose or in extracts from control cells containing the E. coli-yeast shuttle vector without the HSV-1 DNA polymerase gene grown in the presence of raffinose of galactose [15].

Biological context of Genetic Vectors


Anatomical context of Genetic Vectors


Associations of Genetic Vectors with chemical compounds

  • The resistance determinant, portions of pHV2, and an ampicillin- and tetracycline-resistance-conferring pBR322 derivative, pAT153, were ligated together to form the shuttle vectors pWL101 and pWL102 [26].
  • We have investigated the frequency and spectrum of mutations induced when a shuttle vector carrying AAF adducts (85% bound to the C8 position of guanine, 15% to the N2 position) replicated in human cells [27].
  • By using a shuttle vector system developed in our laboratory, we have carried out studies on the molecular mechanism by which 5-bromodeoxyuridine (BrdUrd) induces mutations in mammalian cells [28].
  • By using a shuttle vector system developed in our laboratory, we have analyzed the sequence specificity of mutations induced in mouse A9 cells by exposure of the cells to a high concentration of thymidine [29].
  • To test this hypothesis we introduced the Ha-ras oncogene into SP1 cells on a shuttle vector in which maintenance of the vector was dependent on selection for resistance to the antibiotic G418 [30].

Gene context of Genetic Vectors

  • A GAL4 fragment consisting of residues 1-147 plus two additional residues from the cloning vector [denoted GAL4(149*)] has been cloned and overexpressed in Escherichia coli [31].
  • The GAPDH coding region was detected within a 4.65-kilobase Xho I/EcoRI genomic fragment that was completely sequenced by using the M13 cloning vector system [32].
  • An expression library was constructed by inserting 5' portion-enriched cDNAs from phytohemagglutinin-stimulated peripheral blood mononuclear cells into upstream of signal sequence-deleted CD4 cDNA in an Epstein-Barr virus shuttle vector [33].
  • The rat Kv4.2-coding sequence was truncated at a position just past the first transmembrane segment and subcloned into an adenoviral shuttle vector downstream of a cytomegalovirus promoter (pE1Kv4.2ST) [34].
  • Yeast strains containing the cloned DFR1 gene on multicopy number shuttle vector plasmids show dramatically enhanced methotrexate resistance [35].

Analytical, diagnostic and therapeutic context of Genetic Vectors


  1. In vivo transfer of genetic information between gram-positive and gram-negative bacteria. Trieu-Cuot, P., Gerbaud, G., Lambert, T., Courvalin, P. EMBO J. (1985) [Pubmed]
  2. A positive selection vector for cloning high molecular weight DNA by the bacteriophage P1 system: improved cloning efficacy. Pierce, J.C., Sauer, B., Sternberg, N. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  3. Cloning and heterologous expression of a gene cluster for the biosynthesis of tetracenomycin C, the anthracycline antitumor antibiotic of Streptomyces glaucescens. Motamedi, H., Hutchinson, C.R. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  4. Construction of poxviruses as cloning vectors: insertion of the thymidine kinase gene from herpes simplex virus into the DNA of infectious vaccinia virus. Panicali, D., Paoletti, E. Proc. Natl. Acad. Sci. U.S.A. (1982) [Pubmed]
  5. Site-specific integration by adeno-associated virus is directed by a cellular DNA sequence. Giraud, C., Winocour, E., Berns, K.I. Proc. Natl. Acad. Sci. U.S.A. (1994) [Pubmed]
  6. Evidence for an intron-contained sequence required for the splicing of yeast RNA polymerase II transcripts. Langford, C.J., Gallwitz, D. Cell (1983) [Pubmed]
  7. Glycine 699 is pivotal for the motor activity of skeletal muscle myosin. Kinose, F., Wang, S.X., Kidambi, U.S., Moncman, C.L., Winkelmann, D.A. J. Cell Biol. (1996) [Pubmed]
  8. Functional copies of a human gene can be directly isolated by transformation-associated recombination cloning with a small 3' end target sequence. Kouprina, N., Annab, L., Graves, J., Afshari, C., Barrett, J.C., Resnick, M.A., Larionov, V. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  9. A genetic system for Archaea of the genus Methanosarcina: liposome-mediated transformation and construction of shuttle vectors. Metcalf, W.W., Zhang, J.K., Apolinario, E., Sowers, K.R., Wolfe, R.S. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  10. Structure and function in rhodopsin: expression of functional mammalian opsin in Saccharomyces cerevisiae. Mollaaghababa, R., Davidson, F.F., Kaiser, C., Khorana, H.G. Proc. Natl. Acad. Sci. U.S.A. (1996) [Pubmed]
  11. Efficient recovery and sequencing of mutant genes from mammalian chromosomal DNA. Ashman, C.R., Jagadeeswaran, P., Davidson, R.L. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  12. Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells. Mudgett, J.S., Taylor, W.D. Mol. Cell. Biol. (1990) [Pubmed]
  13. Rapid generation of directed and unmarked deletions in Xanthomonas. Kamoun, S., Tola, E., Kamdar, H., Kado, C.I. Mol. Microbiol. (1992) [Pubmed]
  14. Expression in mammalian cells of a gene from Streptomyces alboniger conferring puromycin resistance. Vara, J.A., Portela, A., Ortín, J., Jiménez, A. Nucleic Acids Res. (1986) [Pubmed]
  15. Expression of herpes simplex virus type 1 DNA polymerase in Saccharomyces cerevisiae and detection of virus-specific enzyme activity in cell-free lysates. Haffey, M.L., Stevens, J.T., Terry, B.J., Dorsky, D.I., Crumpacker, C.S., Wietstock, S.M., Ruyechan, W.T., Field, A.K. J. Virol. (1988) [Pubmed]
  16. Spectra of spontaneous and mutagen-induced mutations in the lacI gene in transgenic mice. Kohler, S.W., Provost, G.S., Fieck, A., Kretz, P.L., Bullock, W.O., Sorge, J.A., Putman, D.L., Short, J.M. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  17. Enhancer activity of light-responsive regulatory elements in the untranslated leader regions of cyanobacterial psbA genes. Li, R., Golden, S.S. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  18. Direct isolation of the functional human thymidine kinase gene with a cosmid shuttle vector. Lau, Y.F., Kan, Y.W. Proc. Natl. Acad. Sci. U.S.A. (1984) [Pubmed]
  19. Multiple point mutations in a shuttle vector propagated in human cells: evidence for an error-prone DNA polymerase activity. Seidman, M.M., Bredberg, A., Seetharam, S., Kraemer, K.H. Proc. Natl. Acad. Sci. U.S.A. (1987) [Pubmed]
  20. Structure of the has operon promoter and regulation of hyaluronic acid capsule expression in group A Streptococcus. Albertí, S., Ashbaugh, C.D., Wessels, M.R. Mol. Microbiol. (1998) [Pubmed]
  21. Recombinant retroviruses that transduce individual polyoma tumor antigens: effects on growth and differentiation. Cherington, V., Morgan, B., Spiegelman, B.M., Roberts, T.M. Proc. Natl. Acad. Sci. U.S.A. (1986) [Pubmed]
  22. Deletions at short direct repeats and base substitutions are characteristic mutations for bleomycin-induced double- and single-strand breaks, respectively, in a human shuttle vector system. Dar, M.E., Jorgensen, T.J. Nucleic Acids Res. (1995) [Pubmed]
  23. Mechanism of mutation on DNA templates containing synthetic abasic sites: study with a double strand vector. Takeshita, M., Eisenberg, W. Nucleic Acids Res. (1994) [Pubmed]
  24. Construction of stable cloning vectors that do not segregate from a human fecal Escherichia coli strain in the streptomycin-treated mouse large intestine. Burghoff, R.L., Laux, D.C., Cohen, P.S. Infect. Immun. (1990) [Pubmed]
  25. Reduced growth factor requirements and accelerated cell-cycle kinetics in adult human melanocytes transformed with SV40 large T antigen. Zepter, K., Häffner, A.C., Trefzer, U., Elmets, C.A. J. Invest. Dermatol. (1995) [Pubmed]
  26. Shuttle vectors for the archaebacterium Halobacterium volcanii. Lam, W.L., Doolittle, W.F. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  27. Replication of acetylaminofluorene-adducted plasmids in human cells: spectrum of base substitutions and evidence of excision repair. Mah, M.C., Boldt, J., Culp, S.J., Maher, V.M., McCormick, J.J. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  28. DNA base sequence changes and sequence specificity of bromodeoxyuridine-induced mutations in mammalian cells. Davidson, R.L., Broeker, P., Ashman, C.R. Proc. Natl. Acad. Sci. U.S.A. (1988) [Pubmed]
  29. Thymidine-induced mutations in mammalian cells: sequence specificity and implications for mutagenesis in vivo. Kresnak, M.T., Davidson, R.L. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  30. Persistence of Ha-ras-induced metastatic potential of SP1 mouse mammary tumors despite loss of the Ha-ras shuttle vector. Schlatter, B., Waghorne, C.G. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
  31. Structure and function of the Zn(II) binding site within the DNA-binding domain of the GAL4 transcription factor. Pan, T., Coleman, J.E. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  32. Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene. Stone, E.M., Rothblum, K.N., Alevy, M.C., Kuo, T.M., Schwartz, R.J. Proc. Natl. Acad. Sci. U.S.A. (1985) [Pubmed]
  33. Molecular cloning of a novel T cell-directed CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. Imai, T., Yoshida, T., Baba, M., Nishimura, M., Kakizaki, M., Yoshie, O. J. Biol. Chem. (1996) [Pubmed]
  34. Suppression of neuronal and cardiac transient outward currents by viral gene transfer of dominant-negative Kv4.2 constructs. Johns, D.C., Nuss, H.B., Marban, E. J. Biol. Chem. (1997) [Pubmed]
  35. Molecular characterization of the Saccharomyces cerevisiae dihydrofolate reductase gene (DFR1). Lagosky, P.A., Taylor, G.R., Haynes, R.H. Nucleic Acids Res. (1987) [Pubmed]
  36. Extent of N-terminal methionine excision from Escherichia coli proteins is governed by the side-chain length of the penultimate amino acid. Hirel, P.H., Schmitter, M.J., Dessen, P., Fayat, G., Blanquet, S. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  37. Decreased electroporation efficiency in Borrelia burgdorferi containing linear plasmids lp25 and lp56: impact on transformation of infectious B. burgdorferi. Lawrenz, M.B., Kawabata, H., Purser, J.E., Norris, S.J. Infect. Immun. (2002) [Pubmed]
  38. Functional cloning vectors for use in directional cDNA cloning using cohesive ends produced with T4 DNA polymerase. Kuijper, J.L., Wiren, K.M., Mathies, L.D., Gray, C.L., Hagen, F.S. Gene (1992) [Pubmed]
  39. Molecular cloning with a pMEA300-derived shuttle vector and characterization of the Amycolatopsis methanolica prephenate dehydratase gene. Vrijbloed, J.W., van Hylckama Vlieg, J., van der Put, N.M., Hessels, G.I., Dijkhuizen, L. J. Bacteriol. (1995) [Pubmed]
  40. The region controlling the thermosensitive effect of plasmid Rts1 on host growth is separate from the Rts1 replication region. Yamamoto, T., Finver, S., Yokota, T., Bricker, J., Kaji, A. J. Bacteriol. (1981) [Pubmed]
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