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Gene Review

ADE1  -  phosphoribosylaminoimidazolesuccinocarboxa...

Saccharomyces cerevisiae S288c

Synonyms: Phosphoribosylaminoimidazole-succinocarboxamide synthase, SAICAR synthetase, YAR015W
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Disease relevance of ADE1


High impact information on ADE1

  • Ade1(+) Leu2(+) transformants were recovered in which the entire LEU2 gene and as much as 7 kb of additional sequences were found on the plasmid, joined by microhomologies characteristic of nonhomologous end-joining (NHEJ) [3].
  • The cDNA that complemented ade2 (phosphoribosylaminoimidazole carboxylase) also complemented ade1 (phosphoribosylaminoimidazole succinocarboxamide synthetase), supporting earlier data suggesting that in some organisms these functions are part of a bifunctional protein [4].
  • Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region [1].
  • The polypeptide fold for residues 204-221 of the E. coli structure differs significantly from those of the ligand-free SAICAR synthetase from Thermatoga maritima and the adenine nucleotide complexes of the synthetase from Saccharomyces cerevisiae [5].
  • RNA prepared from the galactose-induced yeast cultures possesses an activity that cleaves ADE1 mRNA in vitro [6].

Biological context of ADE1


Anatomical context of ADE1


Associations of ADE1 with chemical compounds

  • The enhancement in mutation induced by nitrous acid, UV light, and 6-N-hydroxylaminopurine has been confirmed in a forward-mutation assay (induction of mutations in the ADE1, ADE2 genes) [13].
  • Consistent with this, we find that recombination of a chromosomal ade1 allele with a plasmid-borne ADE1 ORF under the control of the GAL1 promoter increased from 6.1x10(-6) to 1.7x10(-4) when transcription of the plasmid locus was induced by growing the cells in the presence of galactose [14].
  • On ADE1 Bas1p bound the promoter efficiently by itself, but required adenine limitation and Bas2p interaction through BIRD for derepression [15].
  • The ADE1 gene of the yeast Pichia methanolica encodes phosphoribosyl-5-aminoimidazole-carboxylase (AIRC, EC, which is involved in purine biosynthesis [16].
  • The ade1 gene of the fission yeast Schizosaccharomyces pombe encodes a bifunctional polypeptide with glycinamide ribotide synthetase (GARSase) and aminoimidazole ribotide synthetase (AIRSase) enzyme activities [17].

Regulatory relationships of ADE1

  • An expression plasmid containing the ADE1 coding region under control of the PHO5 promoter produced N-succinyl-5-aminoimidazole-4-carboxamide ribotide (SAICAR) synthetase in yeast cells at a level of 40% of total cellular protein [18].

Other interactions of ADE1

  • We describe the isolation and characterization of three new biosynthetic genes-ARG4, ADE1, and URA3-from the methylotrophic yeast Pichia pastoris [19].
  • A new protein kinase gene [called NPK1 (for nonessential protein kinase)] has been found on chromosome I of Saccharomyces cerevisiae between CDC15 and ADE1 [20].
  • Nonselective URA3 colony-color assay in yeast ade1 or ade2 mutants [21].
  • Crossing these three strains with two other ones having markers for ADE1 and pho11::LEU2, we could map the mutation at ca. 4.3 cM and ca. 37.7 cM from the PHO11 and ADE1 loci respectively [22].
  • A two-stage, cyclic fed-batch bioprocess was designed, and its performance evaluated to improve rice alpha-amylase productivity by the yeast Yarrowia lipolytica SMY2 (MatA, ade1, ura3, xpr2), ATCC 201847, containing a replicative plasmid coding for a rice alpha-amlyase [23].

Analytical, diagnostic and therapeutic context of ADE1


  1. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation and analysis of the CEN1-ADE1-CDC15 region. Steensma, H.Y., Crowley, J.C., Kaback, D.B. Mol. Cell. Biol. (1987) [Pubmed]
  2. Cloning and sequencing of a human cDNA coding for a multifunctional polypeptide of the purine pathway by complementation of the ade2-101 mutant in Saccharomyces cerevisiae. Minet, M., Lacroute, F. Curr. Genet. (1990) [Pubmed]
  3. Break-induced replication: a review and an example in budding yeast. Kraus, E., Leung, W.Y., Haber, J.E. Proc. Natl. Acad. Sci. U.S.A. (2001) [Pubmed]
  4. Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations. Schild, D., Brake, A.J., Kiefer, M.C., Young, D., Barr, P.J. Proc. Natl. Acad. Sci. U.S.A. (1990) [Pubmed]
  5. Nucleotide complexes of Escherichia coli phosphoribosylaminoimidazole succinocarboxamide synthetase. Ginder, N.D., Binkowski, D.J., Fromm, H.J., Honzatko, R.B. J. Biol. Chem. (2006) [Pubmed]
  6. Cell cycle arrest promotes trans-hammerhead ribozyme action in yeast. Ferbeyre, G., Bratty, J., Chen, H., Cedergren, R. J. Biol. Chem. (1996) [Pubmed]
  7. Transformation in the methylotrophic yeast Pichia methanolica utilizing homologous ADE1 and heterologous Saccharomyces cerevisiae ADE2 and LEU2 genes as genetic markers. Hiep, T.T., Noskov, V.N., Pavlov, Y.I. Yeast (1993) [Pubmed]
  8. The red ade mutants of Kluyveromyces lactis and their classification by complementation with cloned ADE1 or ADE2 genes from Saccharomyces cerevisiae. Zonneveld, B.J., van der Zanden, A.L. Yeast (1995) [Pubmed]
  9. Isolation and sequencing of a gene, C-ADE1, and its use for a host-vector system in Candida maltosa with two genetic markers. Kawai, S., Hikiji, T., Murao, S., Takagi, M., Yano, K. Agric. Biol. Chem. (1991) [Pubmed]
  10. A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I. Davies, C.J., Hutchison, C.A. Nucleic Acids Res. (1991) [Pubmed]
  11. A hammerhead ribozyme inhibits ADE1 gene expression in yeast. Ferbeyre, G., Bratty, J., Chen, H., Cedergren, R. Gene (1995) [Pubmed]
  12. apd1+, a gene required for red pigment formation in ade6 mutants of Schizosaccharomyces pombe, encodes an enzyme required for glutathione biosynthesis: a role for glutathione and a glutathione-conjugate pump. Chaudhuri, B., Ingavale, S., Bachhawat, A.K. Genetics (1997) [Pubmed]
  13. Saccharomyces cerevisiae mutants with enhanced induced mutation and altered mitotic gene conversion. Ivanov, E.L., Kovaltzova, S.V., Korolev, V.G. Mutat. Res. (1989) [Pubmed]
  14. Stimulation of mitotic recombination upon transcription from the yeast GAL1 promoter but not from other RNA polymerase I, II and III promoters. Bratty, J., Ferbeyre, G., Molinaro, C., Cedergren, R. Curr. Genet. (1996) [Pubmed]
  15. Signaling through regulated transcription factor interaction: mapping of a regulatory interaction domain in the Myb-related Bas1p. Pinson, B., Kongsrud, T.L., Ording, E., Johansen, L., Daignan-Fornier, B., Gabrielsen, O.S. Nucleic Acids Res. (2000) [Pubmed]
  16. The 5-aminoimidazole ribonucleotide-carboxylase structural gene of the methylotrophic yeast Pichia methanolica: cloning, sequencing and homology analysis. Hiep, T.T., Kulikov, V.N., Noskov, V.N., Sizonenko, G.I., Chernoff, Y.O., Pavlov, Y.I. Yeast (1993) [Pubmed]
  17. Sequence of the bifunctional ade1 gene in the purine biosynthetic pathway of the fission yeast Schizosaccharomyces pombe. McKenzie, R., Schuchert, P., Kilbey, B. Curr. Genet. (1987) [Pubmed]
  18. The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control. Myasnikov, A.N., Sasnauskas, K.V., Janulaitis, A.A., Smirnov, M.N. Gene (1991) [Pubmed]
  19. New selectable marker/auxotrophic host strain combinations for molecular genetic manipulation of Pichia pastoris. Lin Cereghino, G.P., Lin Cereghino, J., Sunga, A.J., Johnson, M.A., Lim, M., Gleeson, M.A., Cregg, J.M. Gene (2001) [Pubmed]
  20. NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA. Schweitzer, B., Philippsen, P. Mol. Gen. Genet. (1992) [Pubmed]
  21. Nonselective URA3 colony-color assay in yeast ade1 or ade2 mutants. Weng, Y.S., Nickoloff, J.A. BioTechniques (1997) [Pubmed]
  22. Isolation and characterization of a mutant of Saccharomyces cerevisiae affected in the FLO1 locus. Reboredo, N.M., Sieiro, C., Blanco, P., Villa, T.G. FEMS Microbiol. Lett. (1996) [Pubmed]
  23. Recombinant bioprocess optimization for heterologous protein production using two-stage, cyclic fed-batch culture. Chang, C.C., Ryu, D.D., Park, C.S., Kim, J.Y., Ogrydziak, D.M. Appl. Microbiol. Biotechnol. (1998) [Pubmed]
  24. Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: isolation of the ADE1 gene. Crowley, J.C., Kaback, D.B. J. Bacteriol. (1984) [Pubmed]
  25. Expression in yeast of antisense RNA to ADE1 mRNA. Law, R.H., Devenish, R.J. Biochem. Int. (1988) [Pubmed]
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